驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (IRDye680LT)

ABIN2169649 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Heavy & Light Chain
4745
1877
1842
1022
1020
846
313
283
35
34
31
12
8
7
3
3
2
1
1
1
1
1
1
适用
山羊
2760
2543
2127
1523
1352
732
584
430
383
371
348
339
292
278
225
132
131
113
41
30
29
26
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
宿主

6324
4667
1630
921
519
356
91
67
51
18
8
5
5
5
2
1
1
克隆类型
多克隆
标记
IRDye680LT
2180
1791
1707
1274
768
688
446
297
219
171
165
141
137
129
110
107
75
52
52
49
48
45
41
39
37
35
35
29
28
28
27
27
27
27
27
27
23
18
17
17
15
15
15
15
14
14
14
14
12
12
11
10
10
10
10
10
9
9
9
9
8
8
8
8
8
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
选项
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免疫原 Goat IgG
亚型 IgG
特异性 Goat IgG
Based on ELISA, this antibody reacts with the heavy and light chains of goat IgG and sheep IgG.
交叉反应 绵羊
交叉反应 (详细) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rabbit, rat, chicken, guinea pig, hamster, swine, and horse serum proteins, but may cross-react with immunoglobulins from other species.
产品特性 Blocking buffers and antibody diluents made with bovine serum albumin (BSA) and dry milk may contain IgG that reacts with anti-bovine IgG, anti-goat IgG, anti-horse IgG, and anti-sheep IgG antibodies. This can lead to a significant increase in background and/or reduction of secondary antibody titer for protein detection applications.
纯化方法 Immunoaffinity chromatography
应用备注 Western blot: 1:20,000 Recommended
Optimum dilutions will vary and should be determined empirically.

NOTE: When using PVDF membranes for Western blotting, SDS (final concentration of 0.1 – 0.2%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

  • IRDye 680LT secondary antibodies are suitable for immunofluorescent microscopy and other fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
  • IRDye 680LT is not intended for In-Cell WesternTM assays or in vivo applications.
  • These antibodies are not recommended when getting up and running on system. Once established near-infrared protocols are optimized with IRDye 680RD, IRDye 680LT can be used to optimize signals in the 700 channel. Dilution range 1:20,000 – 1:40,000.
    Note: optimization may be required with IRDye 680LT. Please see pack insert for specific detergents/protocol modifications needed.
  • 说明

    • IRDye 680LT secondary antibodies have been proven to provide the brightest signal for Western blot detection in the 700 nm channel and are comparable to Alexa Fluor® 680 secondary antibodies.
    • Choose IRDye 680LT secondary antibodies to get high signal and for specific uses of detection in the 700nm channel.
    • We do not recommend to start with IRDye 680LT secondaries but rather to start with one of the IRDye 680RD secondaries.

    限制 仅限研究用
    状态 Lyophilized
    溶解方式 Reconstitute with sterile, distilled water
    浓度 1.0 mg/mL
    缓冲液 PBS, pH 7.4
    储存液 Sodium azide
    注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项 Protect from light.
    储存条件 4 °C
    有引用在: Mans, Banz, Mueller, Pabst: "The angiogenesis inhibitor vasostatin is regulated by neutrophil elastase-dependent cleavage of calreticulin in AML patients." in: Blood, Vol. 120, Issue 13, pp. 2690-9, 2012 (PubMed).