驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (IRDye800CW)

ABIN2169607 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Heavy & Light Chain
5119
1885
1842
1055
1039
866
330
304
35
34
31
12
8
7
3
3
2
1
1
1
1
1
1
1
适用
山羊
2914
2617
2172
1556
1392
751
601
456
426
389
365
357
295
280
265
145
132
113
41
30
29
26
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
宿主

6502
4930
1659
1009
521
357
93
67
54
19
8
5
5
5
3
1
1
克隆类型
多克隆
标记
IRDye800CW
2222
1825
1740
1284
772
695
447
307
219
171
165
141
137
129
110
107
77
75
71
65
65
56
55
54
54
53
53
53
53
53
53
52
50
40
37
35
24
19
18
18
17
15
15
15
14
14
14
14
12
12
11
10
10
10
10
10
9
9
9
9
8
8
8
8
8
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
应用范围
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
选项
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品牌 In-Cell Western™,CellTag™
免疫原 Goat IgG, whole molecule
亚型 IgG
特异性 Goat IgG
交叉反应 绵羊
交叉反应 (详细) The antibody also reacts with sheep IgG.
产品特性 IRDye 800CW Donkey anti-Goat IgG is a highly cross-adsorbed secondary antibody suitable for use in a variety of applications including Western blotting, In-Cell Western Assays, In-Gel Westerns, and many others. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG and with the light chains common to most goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Blocking buffers and antibody diluents made with bovine serum albumin (BSA) and dry milk may contain IgG that reacts with anti-bovine IgG, anti-goat IgG, anti-horse IgG, and anti-sheep IgG antibodies. This can lead to a significant increase in background and/or reduction of secondary antibody titer for protein detection applications.
纯化方法 immunoaffinity chromatography
应用备注 Western blot: 1:5,000 - 1:25,000
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitute with sterile, distilled water
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Protect from light.
储存条件 4 °C
有引用在: Li, Li, Sun, Dong, Li, Zhao, Guo, Weng, Qiu: "Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth." in: Journal of virology, Vol. 87, Issue 4, pp. 2072-80, 2013 (PubMed).