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- 抗原 See all CD235ab (CD235a + CD235b) products
- CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD235ab antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- 4AHIR2 monoclonal antibody reactes with CD235, Glycophorins A , which is major sialoglycoproteins of the human erythrocyte membrane. Glycophorins A is a transmembrane dimeric complex of 31 kDa with caboxyterminal ends extending into the cytoplasm of red cells. CD235 antigen is expressed on human red blood cells , normoblasts and erythroid precursor cells. It is also found on erythroid leukemias and some megakaryoblastic leukemias. This antobody is useful in studies of human erythroid-lineage cell development.
- 克隆位点
- 4AHIR2
- 亚型
- IgG2b
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anti-Glycophorin A + B (CD235a + CD235b) (N-Term) antibody
CD235a + CD235b 适用: 人 FACS, IHC (fro), IHC (p), AGG 宿主: 小鼠 Monoclonal HIR2 unconjugated
anti-Glycophorin A + B (CD235a + CD235b) (N-Term) antibody (FITC)CD235a + CD235b 适用: 人 FACS 宿主: 小鼠 Monoclonal HIR2 FITC
anti-Glycophorin A + B (CD235a + CD235b) (N-Term) antibody (PE)CD235a + CD235b 适用: 人 FACS 宿主: 小鼠 Monoclonal HIR2 PE
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))
- Abstract
- CD235a + CD235b 产品
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