CA12 抗体 (AA 161-318)
(1 validation)-
- 抗原 See all CA12 抗体
- CA12 (Carbonic Anhydrase 12 (CA12))
-
抗原表位
- AA 161-318
-
适用
- 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This CA12 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Polyclonal Antibody to Carbonic Anhydrase XII (CA12)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against CA12. It has been selected for its ability to recognize CA12 in immunohistochemical staining and western blotting.
- 交叉反应
- 人, 大鼠
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Carbonic Anhydrase XII (CA12)
- 亚型
- IgG
- Top Product
- Discover our top product CA12 Primary Antibody
-
anti-Carbonic Anhydrase 12 (CA12) (AA 154-307) antibody
CA12 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase 12 (CA12) (AA 25-124) antibodyCA12 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Carbonic Anhydrase 12 (CA12) (AA 25-301) antibodyCA12 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase 12 (CA12) (AA 1-354) antibodyCA12 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase 12 (CA12) antibodyCA12 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 4D1 unconjugated
anti-Carbonic Anhydrase 12 (CA12) (AA 241-340) antibodyCA12 适用: 人 ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase 12 (CA12) antibodyCA12 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal CL0280 unconjugated
anti-Carbonic Anhydrase 12 (CA12) antibodyCA12 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal CL0278 unconjugated
anti-Carbonic Anhydrase 12 (CA12) (AA 188-199) antibodyVerified CA12 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Carbonic Anhydrase 12 (CA12) antibodyCA12 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 2E9 unconjugated
-
- 应用备注
-
Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- 说明
-
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
-
- by
- School of Clinical Sciences, University of Bristol
- No.
- #100909
- 日期
- 2017.02.21
- 抗原
- CA12
- Lot Number
- A20170109527
- Method validated
- Immunohistochemistry
- Positive Control
- mouse kidney, mouse ciliary body
- Negative Control
- secondary antibody only
- Notes
- Primary Antibody
- ABIN3204699
- Secondary Antibody
- goat anti-rabbit IgG Alexa Fluor 488 antibody (Life Technology, A-11006, lot 1348202)
- Full Protocol
- Cryo-section:
- Wash mouse eye and kidney tissue in PBS.
- Cryo-protection. Suspend tissue in Optimum Cutting Temperature (OCT) embedding media (Thermo Scientific, Lot 03801137).
- Gently freeze suspended tissue in liquid nitrogen for 5min.
- Sections performed on a cryostat (LEICA, CM3050S) set to 12µm.
- Fix sections in 4% paraformaldehyde (PFA) for 5min at room temperature.
- Rinse sections in PBS 3 times for 5min.
- Block sections with 5% normal goat serum for 1h at RT.
- Immunostaining:
- Incubate sections with primary rabbit-anti CA12 antibody (antibodies-online, ABIN3204699, lot A20170109527) diluted 1:100 in PBSA, overnight at 4°C. Include a negative control without primary antibody and a kidney positive control.
- Rinse sections with PBS 3 times for 5min. Keep negative control in a separate jar.
- Incubate sections with secondary goat anti-rabbit IgG Alexa Fluor 488 antibody conjugate (Life Technology, A-11006, lot 1348202) diluted 1:500 in 1% Bovine Serum Albumin in PBS (PBSA) buffer for 2h at RT.
- Counterstain with DAPI in the mounting medium step.
- Wash and mount:
- Rinse sections in PBS 3 times for 5min.
- Mount sections in Vectorshield mounting medium with DAPI (Vector laboratories, H-1200, lot ZC0814).
- Microscopy:
- Image acquisition on an EVOS FL Color fluorescence and transmitted light microscope (Life technologies, Cat no. AMEFC4300) at 20x magnification.
- Experimental Notes
生效 #100909 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Immunostaining of murine kidney tissue (A.) and ciliary body sections (B.) with the CA12 antibody ABIN3204699 (green; counterstain with DAPI, blue) as described in the protocol. No staining of the plasma membrane is observed in ciliary body section stains with the secondary antibody alone (C.).]()
Immunostaining of murine kidney tissue (A.) and ciliary body sections (B.) with the CA12 antibody ABIN3204699 (green; counterstain with DAPI, blue) as described in the protocol. No staining of the plasma membrane is observed in ciliary body section stains with the secondary antibody alone (C.).
Full Methods -
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
-
- 抗原
- CA12 (Carbonic Anhydrase 12 (CA12))
- 别名
- Carbonic Anhydrase XII (CA12 产品)
- 别名
- CAXII antibody, HsT18816 antibody, CAR12 antibody, 2310047E01Rik antibody, AI314958 antibody, Ca12 antibody, ca12 antibody, carbonic anhydrase 12 antibody, carbonic anhydrase XII L homeolog antibody, CA12 antibody, Car12 antibody, ca12.L antibody
- 背景
- CA-XII, CAXII, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3
-
