LIMK2 抗体 (pThr505)
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- 抗原 See all LIMK2 抗体
- LIMK2 (LIM Domain Kinase 2 (LIMK2))
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抗原表位
- pThr505
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LIMK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of LIMK2 only when phosphorylated at Threonine 505.
- 纯化方法
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- 免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
- Top Product
- Discover our top product LIMK2 Primary Antibody
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anti-LIM Domain Kinase 2 (LIMK2) (pSer283) antibody
LIMK2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 2 (LIMK2) (AA 1-686) antibodyLIMK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2H2-E11 unconjugated
anti-LIM Domain Kinase 2 (LIMK2) (pThr505) antibodyLIMK2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 2 (LIMK2) (AA 1-686) antibodyLIMK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 2 (LIMK2) antibodyLIMK2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 2 (LIMK2) (Ser283) antibodyLIMK2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 2 (LIMK2) (AA 1-240) antibodyLIMK2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Kinase 2 (LIMK2) (AA 1-686) antibodyLIMK2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-LIM Domain Kinase 2 (LIMK2) (AA 181-193) antibodyVerified LIMK2 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-LIM Domain Kinase 2 (LIMK2) (AA 1-240) antibodyLIMK2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at -20 °C.
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- 抗原
- LIMK2 (LIM Domain Kinase 2 (LIMK2))
- 别名
- LIMK2 (LIMK2 产品)
- 别名
- xlimk2 antibody, zgc:91990 antibody, wu:fa97c09 antibody, wu:fk73e11 antibody, LIMK2 antibody, Limk2b antibody, Link2 antibody, LIMK antibody, 9430059K01 antibody, A930024P04Rik antibody, C85310 antibody, Limk2a antibody, LIM domain kinase 2 L homeolog antibody, LIM domain kinase 2 antibody, LIM motif-containing protein kinase 2 antibody, limk2.L antibody, limk2 antibody, LIMK2 antibody, Limk2 antibody
- 背景
- LIM kinases (LIM kinase 1 and LIM kinase 2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs in their N terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIM kinase 1 and LIM kinase 2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N terminus of Cofilin.Synonyms: EC=2.7.11.1, LIM domain kinase 2, LIMK-2, LIMK2
- 基因ID
- 3985
- NCBI登录号
- NP_005560
- UniProt
- P53671
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