FOXP3 抗体 (Forkhead Box P3)

Details for Product anti-FOXP3 Antibody No. ABIN188539, 供应商: Log in to see
抗原
  • foxp3
  • FOXP3
  • AIID
  • DIETER
  • IPEX
  • PIDX
  • XPID
  • JM2
  • scurfin
  • sf
  • RGD1562112
  • forkhead box P3
  • forkhead box P3a
  • forkhead box P3 L homeolog
  • FOXP3
  • foxp3a
  • foxp3
  • foxp3.L
  • Foxp3
适用
人, 小鼠, 大鼠
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宿主

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3
克隆类型
多克隆
标记
This FOXP3 antibody is un-conjugated
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应用范围
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
纯化方法 Epitope affinity purified
别名 FoxP3 (FOXP3 Antibody 摘要)
背景 Gene Symbol: FOXP3
基因ID 50943
UniProt Q99JB6
途径 Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
应用备注 Western Blot 1:2000, Flow Cytometry 1:8000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 1:400This FOXP3 antibody can be used for Immunoprecipitation, Immunohistochemistry paraffin embeded sections and Western blot analysis, where a band is seen ~48 kDa in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. In IP transfected FOXP3-HA tagged proteins were used. In IHC-P, staining was observed in the nuclei of mouse spleen cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended.
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

实验流程 Immunohistochemistry-Paraffin Embedded Sections NB100-39002 Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 degrees C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL
缓冲液 PBS
Buffer contains: 0.05 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze-thaw cycles
储存条件 -80 °C,-20 °C
储存方法 Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
厂商提供的图像
Western Blotting (WB) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Western Blot: FOXP3 Antibody [ABIN1885392] - Detection of immunoprecipitated FOXP3 fr...
Immunohistochemistry (IHC) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunohistochemistry: FOXP3 Antibody [ABIN1885392] - FOXP3 antibody was tested in mou...
Flow Cytometry (FACS) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Flow Cytometry: FOXP3 Antibody [ABIN1885392] - Transfectants from A were permeabilize...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunohistochemistry-Paraffin: FoxP3 Antibody [ABIN1885392] - Mouse spleen stained wi...
Western Blotting (WB) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Western Blot: FOXP3 Antibody [ABIN1885392] - Jurkat Tag cells were transfected with t...
Immunofluorescence (IF) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunocytochemistry/Immunofluorescence: FOXP3 Antibody [ABIN1885392] - The same trans...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunohistochemistry-Paraffin: FoxP3 Antibody - IHC analysis of a formalin fixed par...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Forkhead Box P3 (FOXP3) antibody (ABIN188539) Immunohistochemistry-Paraffin: FoxP3 Antibody - IHC analysis of a formalin fixed par...
有引用在: Busch, Annamalai, Abdusalamova, Reichhart, Huber, Lin, Jo, Zipfel, Skerka, Wildner, Diedrichs-Möhring, Rohrer, Strauß: "Anaphylatoxins Activate Ca(2+), Akt/PI3-Kinase, and FOXO1/FoxP3 in the Retinal Pigment Epithelium." in: Frontiers in immunology, Vol. 8, pp. 703, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).

Varga, Piazuelo, Romero-Gallo, Delgado, Suarez, Whitaker, Krishna, Patel, Skaar, Wilson, Algood, Peek: "TLR9 activation suppresses inflammation in response to Helicobacter pylori infection." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 311, Issue 5, pp. G852-G858, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Zhang, Yao, Wang, Yang, Wang, Fan, Harris: "Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension." in: The Journal of clinical investigation, Vol. 125, Issue 11, pp. 4281-94, 2015 (PubMed).

Kreiter, Vormehr, van de Roemer, Diken, Löwer, Diekmann, Boegel, Schrörs, Vascotto, Castle, Tadmor, Schoenberger, Huber, Türeci, Sahin: "Mutant MHC class II epitopes drive therapeutic immune responses to cancer." in: Nature, Vol. 520, Issue 7549, pp. 692-6, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Rajnai, Heyning, Koens, Sebestyén, Andrikovics, Hogendoorn, Matolcsy, Szepesi: "The density of CD8+ T-cell infiltration and expression of BCL2 predicts outcome of primary diffuse large B-cell lymphoma of bone." in: Virchows Archiv : an international journal of pathology, Vol. 464, Issue 2, pp. 229-39, 2014 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Grant, Jung, Johnson, Kostakoglu, Hsi, Byrd, Jones, Leonard, Martin, Cheson: "A phase 2 trial of extended induction epratuzumab and rituximab for previously untreated follicular lymphoma: CALGB 50701." in: Cancer, Vol. 119, Issue 21, pp. 3797-804, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Sun, Lian, Ma, Wang, Ma, Wang, Zhai, Meng, Feng, Bai, Cui, Fang: "Competing endogenous RNA network analysis of CD274, IL‑10 and FOXP3 co‑expression in laryngeal squamous cell carcinoma." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 3859-3869, 1970 (PubMed). Method employed by authors: Immunohistochemistry (IHC)