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- 抗原 See all His Tag 抗体
- His Tag
- 适用
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This His Tag antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB)
- 序列
- HHHHHH
- 特异性
- His-tag antibody [Biotin], pAb, Rabbit specifically reacts with fusion proteins containing His tagged fusion proteins.
- 产品特性
- His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. It is suitable for detecting the expression level of His tagged proteins. His-tag antibody [Biotin], pAb, Rabbit is the biotin conjugated Rabbit anti His antibody. The antibody recognizes fusion protein with His tag which is located at either C-terminal, or internal regions of the protein. It is suitable for detecting the expression level of His tagged proteins.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- His epitope tag peptide HHHHHH conjugated to KLH.
- 亚型
- IgG
- Top Product
- Discover our top product His Tag Primary Antibody
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anti-His Tag antibody
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibody适用: 标记, 人 WB 宿主: 小鼠 Monoclonal 2D5 unconjugated
anti-His Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP
anti-His Tag (C-Term) antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag (N-Term) antibody (Biotin)WB, ELISA, IP, FACS, ICC, IHC (p) 宿主: 兔 Monoclonal RM146 Biotin
anti-His Tag (N-Term) antibodyWB, IP, FACS, ICC, IHC (p) 宿主: 兔 Monoclonal RM146 unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0. 2-1 µg/mL - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
- 浓度
- 0.5 mg/mL
- 缓冲液
- lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
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: "Functional analysis of the Autographa californica multiple nucleopolyhedrovirus GP64 terminal fusion loops and interactions with membranes." in: Journal of virology, Vol. 86, Issue 18, pp. 9617-28, (2012) (PubMed).
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: "Functional analysis of the Autographa californica multiple nucleopolyhedrovirus GP64 terminal fusion loops and interactions with membranes." in: Journal of virology, Vol. 86, Issue 18, pp. 9617-28, (2012) (PubMed).
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- 抗原
- His Tag
- Abstract
- His Tag 产品
- 物质类
- Tag
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