GAD65 抗体 (AA 3-96)
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- 抗原 See all GAD65 (GAD2) 抗体
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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抗原表位
- AA 3-96
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适用
- 大鼠, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GAD65 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 特异性
- Specific for GAD 2 / GAD 65
- 纯化方法
- purified IgG. Azide was added before lyophilization.
- 免疫原
- Purified recombinant mouse GAD 2 (aa 3-96).
- 克隆位点
- 26H1
- 亚型
- IgG3
- Top Product
- Discover our top product GAD2 Primary Antibody
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- 应用备注
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WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200 up to 1 : 500 - 说明
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WB: This antibody is less sensitive than the rabbit antibody. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742419 as detector antibody (protocol for sandwich-ELISA).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS
- 注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).
: "The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 37, pp. 12289-303, (2014) (PubMed).
: "Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons." in: Neuroscience, Vol. 246, pp. 451-67, (2013) (PubMed).
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Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).
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- 抗原
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- 别名
- GAD 2 (GAD2 产品)
- 别名
- GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody
- 背景
- Synonyms: GAD 65
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