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FZD3 抗体 (AA 121-220)

Name: FZD3 抗体 (AA 121-220)
SKU: ABIN1714171
Availability: OutOfStock

FZD3 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (ABIN1714171) at 1:300 overnight at 4 °C.

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated ( ) at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated-Cy)and used at 1:200 dilution for 40 minutes at 37°CDAPI(5ug/ml) was used to stain the cell nuclei

IF (p)

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated-Cy3)used at 1:200 dilution for 40 minutes at 37°C.

IF (p)

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC (p)

4 images

(1 reference)

产品编号 ABIN1714171

发货至: 中国

Datasheet as PDF

抗原 See all FZD3 抗体

FZD3 (Frizzled Family Receptor 3 (FZD3))
抗原表位
14

8

8

6

4

4

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1
AA 121-220
适用
58

38

23

14

6

6

6

6

2

2

2

2

1

1
人, 小鼠, 大鼠, 非洲爪蟾
宿主
51

4

3

3
兔
Compatible Secondaries
克隆类型
55

6
多克隆
标记
30

5

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This FZD3 antibody is un-conjugated
应用范围
57

30

11

10

6

4

3

2

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human FZD3/Frizzled 3

亚型

IgG
anti-Frizzled Family Receptor 3 (FZD3) (AA 23-205) antibody
FZD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (N-Term) antibody
FZD3 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 小鸡, Hamster, 马, 猴, 兔, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibody
FZD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B8 unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibody
FZD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2H5 unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (Internal Region) antibody
FZD3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibody
FZD3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 1-62) antibody
FZD3 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 581-596), (C-Term), (Extracellular) antibody
FZD3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 481-530) antibody
FZD3 适用: 人, 小鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (N-Term) antibody
FZD3 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 小鸡, Hamster, 马, 猴, 兔, Gorilla IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Su, Zhang, Gan, Jiang, Xiao, Zou, Li, Jiang: "Deregulation of the planar cell polarity genes CELSR3 and FZD3 in Hirschsprung disease." in: Experimental and molecular pathology, Vol. 101, Issue 2, pp. 241-248, (2016) (PubMed).
抗原

FZD3 (Frizzled Family Receptor 3 (FZD3))

别名

FZD3/Frizzled 3 (FZD3 产品)

别名

Fz-3 antibody, FZ-3 antibody, fz3 antibody, Xfz3 antibody, frz3 antibody, hfz3 antibody, frz-3 antibody, frizzled3 antibody, frizzled-3 antibody, FZD3 antibody, AU020229 antibody, D930050A07Rik antibody, Fz3 antibody, fz9 antibody, fzd3 antibody, zg09 antibody, fzd3l antibody, frizzled class receptor 3 antibody, frizzled class receptor 3 L homeolog antibody, frizzled class receptor 3a antibody, frizzled class receptor 3b antibody, FZD3 antibody, fzd3 antibody, fzd3.L antibody, Fzd3 antibody, fzd3a antibody, fzd3b antibody

背景

Synonyms: Fz-3, Frizzled-3, hFz3, FZD3
Background: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

基因ID

7976

UniProt

Q9NPG1

途径

WNT signaling, Tube Formation