ELTD1 抗体 (AA 301-400)
(1 validation)-
- 抗原 See all ELTD1 抗体
- ELTD1 (EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1))
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抗原表位
- AA 301-400
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适用
- 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ELTD1 antibody is un-conjugated
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 小鼠, 大鼠
- 预测反应
- Human,Horse
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from human ETL/ELTD1
- 亚型
- IgG
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anti-EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1) (AA 85-299) antibody
ELTD1 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1) (AA 192-292) antibodyELTD1 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1) (C-Term) antibodyELTD1 适用: 人, 小鼠, 大鼠, 小鸡, 马, Pig, 兔, Bat IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1) antibodyELTD1 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal CL4164 unconjugated
anti-EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1) (N-Term) antibodyELTD1 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1) (C-Term) antibodyELTD1 适用: 人, 小鼠, 大鼠, 小鸡, 马, Pig, 兔, Gorilla IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1) (AA 110-406) antibodyELTD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - 限制
- 仅限研究用
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- by
- Memorial Sloan-Kettering Cancer Center, Parada lab
- No.
- #100001
- 日期
- 2015.10.15
- 抗原
- ELTD1
- Lot Number
- Method validated
- Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
- Positive Control
- Tumor cell transplanted mouse brain was dissected
- Negative Control
- Notes
- The staining pattern was as expected for ELTD1 as membrane protein.
- Primary Antibody
- ABIN1713780
- Secondary Antibody
- Donkey-anti-Rabbit-Cy3
- Full Protocol
- Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
- Immunostaining for Cryo Sections:
- Dry slides at 50-55°C for 15-30 min (or RT for 30min)
- Wash three times with PBS 5min (shake gently)
- Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
- Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
- Rabbit-anti-ELTD1 antibody (ABIN1713780) diluted 1:50-1:400
- Wash three times with PBS 5min (shake gently)
- Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
- Donkey-anti-Rabbit-Cy3
- DAPI 1:100
- Wash three times with PBS 5min (shake gently)
- Add three drops of water-based Immu-mount
- Cover with 24x60 cover glass
- Imaging
- Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
- Experimental Notes
- ABIN1713780 worked within the diution range 1:50 - 1:200; the antibody did not work well at a 1:400 dilution.
生效 #100001 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF validation of ELTD1 antibody ABIN1713780 on mouse brain tumor sections. A. ABIN1713780 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.]()
IF validation of ELTD1 antibody ABIN1713780 on mouse brain tumor sections. A. ABIN1713780 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- ELTD1 (EGF, Latrophilin and Seven Transmembrane Domain Containing 1 (ELTD1))
- 别名
- ETL/ELTD1 (ELTD1 产品)
- 别名
- ELTD1 antibody, ETL antibody, KPG_003 antibody, Etl antibody, wu:fd10a02 antibody, zgc:63629 antibody, 1110033N21Rik antibody, ETL1 antibody, adhesion G protein-coupled receptor L4 antibody, ADGRL4 antibody, Adgrl4 antibody, adgrl4 antibody
- 背景
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Synonyms: ETL, ELTD1, KPG_003, Adhesion G protein-coupled receptor L4, EGF, latrophilin and seven transmembrane domain-containing protein 1, EGF-TM7-latrophilin-related protein, ETL protein, ADGRL4, UNQ202/PRO228
Background: Endothelial orphan receptor that acts as a key regulator of angiogenesis.
- 基因ID
- 64123
- UniProt
- Q9HBW9
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