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ARHGAP22 抗体 (phosphorylated)

ARHGAP22 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FM 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1607879
发货至: 中国
  • 抗原 See all ARHGAP22 抗体
    ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))
    抗原表位
    • 10
    • 8
    • 6
    • 1
    • 1
    • 1
    • 1
    phosphorylated
    适用
    人, 小鼠, 大鼠
    宿主
    • 28
    克隆类型
    • 28
    多克隆
    标记
    • 11
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ARHGAP22 antibody is un-conjugated
    应用范围
    • 21
    • 15
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
    交叉反应 (详细)
    Cross reactivity with ARHGAP22 pS397 from other sources has not been determined.
    纯度
    Anti-ARHGAP22 pS397 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for phosphorylated ARHGAP22 at Serine 397. It also recognizes the S22-> A mutation but not the S397-> A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100 % sequence homology.
    内毒素水平
    Low Endotoxin : No
    免疫原
    ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the region surrounding mouse pS397 region of ARHGAP22.
    Immunogen Type: Peptide
    Top Product
    Discover our top product ARHGAP22 Primary Antibody
  • 应用备注
    Anti-ARHGAP22 pS397 antibody is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.
    ELISA Dilution: 1:20.000 - 1:60.000
    Immunohistochemistry Dilution: 1:100-1:500
    IF Microscopy Dilution: 1:100-1:500
    Western Blot Dilution: 1 µg/mL
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.07 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 50 % (v/v) Glycerol
    储存条件
    4 °C/-20 °C
    储存方法
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    Expiration date is six (6) months from date of opening.
  • 抗原
    ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))
    别名
    ARHGAP22 (ARHGAP22 产品)
    别名
    si:ch211-223a10.2 antibody, RhoGAP2 antibody, RhoGap22 antibody, B230341L19Rik antibody, RHOGAP2 antibody, p68RacGAP antibody, Rho GTPase activating protein 22 antibody, rho GTPase activating protein 22 antibody, ARHGAP22 antibody, arhgap22 antibody, CC1G_06014 antibody, Arhgap22 antibody
    背景
    ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser397] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research. Synonyms: Rho-type GTPase-activating protein 22, RHOGAP2, RhoGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22
    基因ID
    239027
    NCBI登录号
    NP_722495
    UniProt
    Q8BL80
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