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V5 Epitope Tag 抗体 (HRP)

WB, ELISA 宿主: 兔 Polyclonal HRP
产品编号 ABIN1573948
发货至: 中国
  • 抗原 See all V5 Epitope Tag products
    V5 Epitope Tag
    适用
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    克隆类型
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    This V5 Epitope Tag antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), ELISA
    序列
    GKPIPNPLLG LDST
    特异性
    Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
    纯化方法
    Immunoaffinity chromatography
    免疫原
    V5 epitope tag peptide GKPIPNPLLGLDST conjugated - KLH
    亚型
    IgG
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Other applications: user-optimized
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal
    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Dahl, Corydon, Ränkel, Nielsen, Füchtbauer, Knudsen: "An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus." in: Cancer cell international, Vol. 14, Issue 1, pp. 17, (2014) (PubMed).

    Tahir, Yang, Goltsov, Song, Ren, Wang, Chang, Thompson: "Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer." in: Cancer research, Vol. 73, Issue 6, pp. 1900-11, (2013) (PubMed).

    Zhang, Li, Jaiswal, Bayat, Xiong, Sandoval, Charng, David, Haueter, Yamamoto, Graham, Bellen: "The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 807-20, (2013) (PubMed).

  • 抗原
    V5 Epitope Tag
    别名
    V5-Tag (V5 Epitope Tag 产品)
    物质类
    Tag
    背景
    Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
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