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RFP 抗体

Name: RFP 抗体
Brand: antibodies-online
SKU: ABIN1573925
Availability: OutOfStock

RFP 适用: Discosoma WB, ELISA 宿主: 兔 Polyclonal unconjugated

Loading: RFP-tag fusion protein expressed in E.

2 images

(5 references)

产品编号 ABIN1573925

发货至: 中国

Datasheet as PDF

抗原 See all RFP 抗体

RFP (Red Fluorescent Protein (RFP))

适用

Discosoma
宿主
23

13

9

5

3
兔
Compatible Secondaries
克隆类型
30

23
多克隆
标记
19

5

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RFP antibody is un-conjugated
应用范围
27

21

15

12

10

7

6

6

2

1

1

1
Western Blotting (WB), ELISA

原理

RFP-tag Antibody, pAb, Rabbit

序列

VNGHEFEIEGEGEGR

特异性

Rabbit Anti-RFP-tag Polyclonal Antibody specifically reacts with fusion proteins containing RFP-tags. The antibody does not react with GFP or GFP-tagged proteins.

交叉反应（详细）

RFP-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. The antibody does not react with GFP or GFP-tagged proteins.

纯化方法

Immunoaffinity chromatography

免疫原

RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated - KLH

亚型

IgG

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anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated

anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated

anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Red Fluorescent Protein (RFP) antibody (Biotin)
RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal Biotin

anti-Red Fluorescent Protein (RFP) antibody (Biotin)
RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal Biotin

anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633

anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488

anti-Red Fluorescent Protein (RFP) antibody (HRP)
RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal HRP

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freezing and thawing cycles.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
Alvarez-Guaita, Vilà de Muga, Owen, Williamson, Magenau, García-Melero, Reverter, Hoque, Cairns, Cornely, Tebar, Grewal, Gaus, Ayala-Sanmartín, Enrich, Rentero: "Evidence for annexin A6-dependent plasma membrane remodelling of lipid domains." in: British journal of pharmacology, Vol. 172, Issue 7, pp. 1677-90, (2015) (PubMed).

Yang, Shen, Garre, Hao, Krumlinde, Cvijovi?, Arens, Nyström, Liu, Sunnerhagen: "Stress granule-defective mutants deregulate stress responsive transcripts." in: PLoS genetics, Vol. 10, Issue 11, pp. e1004763, (2014) (PubMed).

Devers, Teply, Reinert, Gaude, Krajinski: "An endogenous artificial microRNA system for unraveling the function of root endosymbioses related genes in Medicago truncatula." in: BMC plant biology, Vol. 13, pp. 82, (2013) (PubMed).

Roberts, Baines: "Myosin Va enhances secretion of herpes simplex virus 1 virions and cell surface expression of viral glycoproteins." in: Journal of virology, Vol. 84, Issue 19, pp. 9889-96, (2010) (PubMed).

Nilsson, Sunnerhagen: "Cellular stress induces cytoplasmic RNA granules in fission yeast." in: RNA (New York, N.Y.), Vol. 17, Issue 1, pp. 120-33, (2010) (PubMed).
抗原

RFP (Red Fluorescent Protein (RFP))

别名

RFP-Tag (RFP 产品)

背景

Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.