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HAT-Tag 抗体

ELISA, WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1573907
发货至: 中国
  • 抗原
    HAT-Tag
    适用
    请咨询
    宿主
    • 2
    • 1
    克隆类型
    • 2
    • 1
    多克隆
    标记
    • 2
    • 1
    非结合性
    应用范围
    • 3
    • 3
    • 2
    ELISA, Western Blotting (WB)
    序列
    KDHLIHNVHK EFHAHAHNK
    特异性
    Rabbit Anti-HAT-tag Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal HAT tagged fusion proteins.
    纯化方法
    Immunoaffinity chromatography
    免疫原
    HAT epitope tag peptide KDHLIHNVHKEFHAHAHNK conjugated - KLH
    亚型
    IgG
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 5 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    PBS, pH7.4, containing 0.02 % sodium azid
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    储存条件
    4 °C/-20 °C
    储存方法
    The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Chandrasekera, Wan, Gizewski, Auchampach, Lasley: "Adenosine A1 receptors heterodimerize with ?1- and ?2-adrenergic receptors creating novel receptor complexes with altered G protein coupling and signaling." in: Cellular signalling, Vol. 25, Issue 4, pp. 736-42, (2013) (PubMed).

    Okada, Yamaura, Suzuki, Itoh, Osada, Takahashi: "Molecular characterization and expression analysis of chitinase from the Pacific oyster Crassostrea gigas." in: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 165, Issue 2, pp. 83-9, (2013) (PubMed).

    Xiao, Zhou, Sun, Feng, Du, Gao, Ji, Yang, Wang, Han, Langford, Lei: "Apa is a trimeric autotransporter adhesin of Actinobacillus pleuropneumoniae responsible for autoagglutination and host cell adherence." in: Journal of basic microbiology, Vol. 52, Issue 5, pp. 598-607, (2012) (PubMed).

  • 抗原
    HAT-Tag
    物质类
    Tag
    背景
    Rabbit Anti-HAT-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.
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