Podocalyxin-Like (PODXL) 抗体

ABIN151343 产品详细信息, 供应商: Log in to see
抗原
  • Gp200
  • PC
  • PCLP
  • PCLP-1
  • AW121214
  • Ly102
  • Pclp1
  • podocalyxin
  • MEP21
  • PODXL
  • Gp135
  • PCLP1
  • cPCLP1
  • podxl
  • fc18d02
  • wu:fc18d02
  • Podxl
  • podocalyxin like
  • podocalyxin-like
  • PODXL
  • Podxl
  • podxl
适用

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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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应用范围
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 Human embryonal carcinoma cell line 2102Ep.
克隆位点 TRA-1-81
亚型 IgM
无交叉反应 小鼠
纯化方法 IgM purified
抗原 Podocalyxin-Like (PODXL)
别名 TRA-1-81 (PODXL Antibody 摘要)
途径 Tube Formation
应用备注 Western Blot 1:500, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200This TRA-1-81 antibody is useful for Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot. Flow Cytometry was reported in scientific literature.
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

实验流程 Protocol Specific for NB100-1833 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze-thaw cycles
储存条件 4 °C,-20 °C
储存方法 Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
厂商提供的图像
Western Blotting (WB) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN151343) Western Blot: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Western blot analysis of AB...
Flow Cytometry (FACS) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN151343) Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Indirect FCM analysis of ...
Immunofluorescence (IF) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN151343) Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Immunofluorescence st...
Flow Cytometry (FACS) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN151343) Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Analysis using the Alexa ...
有引用在: Rossbach, Hildebrand, El-Ahmad, Stachelscheid, Reinke, Kurtz: "Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector." in: Stem cell research, Vol. 16, Issue 2, pp. 314-7, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Hildebrand, Seemann, Kurtz, Hecht, Contzen, Gossen, Stachelscheid: "Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses." in: Cellular and molecular life sciences : CMLS, Vol. 72, Issue 23, pp. 4671-80, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Mizrak, Chikhovskaya, Sadri-Ardekani, van Daalen, Korver, Hovingh, Roepers-Gajadien, Raya, Fluiter, de Reijke, de la Rosette, Knegt, Belmonte, van der Veen, de Rooij, Repping, van Pelt: "Embryonic stem cell-like cells derived from adult human testis." in: Human reproduction (Oxford, England), Vol. 25, Issue 1, pp. 158-67, 2009 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Henderson, Draper, Baillie, Fishel, Thomson, Moore, Andrews: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Flow Cytometry,Immunofluorescence

Badcock, Pigott, Goepel, Andrews: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999 (PubMed). (Sample species: Human). Further details: Western Blotting

Rossbach, Hildebrand, El-Ahmad, Stachelscheid, Reinke, Kurtz: "Corrigendum to "Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector" [Stem Cell Res. 16/2 (2016) 314-317]." in: Stem cell research, Vol. 17, Issue 2, pp. 461, 1970 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Rossbach, Hildebrand, El-Ahmad, Stachelscheid, Reinke, Kurtz: "Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using integration free Sendai virus technology." in: Stem cell research, Vol. 21, pp. 167-170, 1970 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

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