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DEPTOR 抗体 (AA 56-170)

Name: DEPTOR 抗体 (AA 56-170)
SKU: ABIN1387597
Availability: OutOfStock

DEPTOR 适用: 人, 小鼠, 大鼠 WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with DEPTOR Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti DEPTOR/DEPDC6 Polyclonal Antibody, Unconjugated (ABIN1387597) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin embedded rat liver labeled with Anti DEPTOR/DEPDC6 Polyclonal Antibody, Unconjugated (ABIN1387597) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with DEPTOR Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

4 images

(2 references)

产品编号 ABIN1387597

发货至: 中国

Datasheet as PDF

抗原 See all DEPTOR 抗体

DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))
抗原表位
15

14

5

1

1

1

1

1

1

1

1

1

1

1

1
AA 56-170
适用
46

33

18

3

2

2

2

2

2

2

1
人, 小鼠, 大鼠
宿主
41

4

1
兔
Compatible Secondaries
克隆类型
44

2
多克隆
标记
18

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This DEPTOR antibody is un-conjugated
应用范围
44

25

25

9

7

5

2

2

1
Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Pig,Horse,Chicken,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human DEPTOR/DEPDC6

亚型

IgG
anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 1-409) antibody
DEPTOR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 1-220) antibody
DEPTOR 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 86-120) antibody
DEPTOR 适用: 人 WB 宿主: 兔 Polyclonal RB57442 unconjugated

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 3-38) antibody
DEPTOR 适用: 人 WB 宿主: 兔 Polyclonal RB57441 unconjugated

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 1-409) antibody
DEPTOR 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 1-409) antibody
DEPTOR 适用: 人 ELISA 宿主: 小鼠 Monoclonal 5B3 unconjugated

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 230-280) antibody
DEPTOR 适用: 人, 大鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 151-250) antibody
DEPTOR 适用: 人, 小鼠 WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 151-250) antibody (Biotin)
DEPTOR 适用: 人, 小鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal Biotin

anti-DEP Domain Containing mTOR-Interacting Protein (DEPTOR) (AA 56-170) antibody (Biotin)
DEPTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal Biotin

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Liu, Cai, Zhang, Liu, Luo, Xing, Du: "Deacetylation of NAT10 by Sirt1 promotes the transition from rRNA biogenesis to autophagy upon energy stress." in: Nucleic acids research, Vol. 46, Issue 18, pp. 9601-9616, (2019) (PubMed).

Liu, Zhang, Liu, Li, Zhang, Li, Liu, Huang, Shen, Gu, Gao, Wu, Wu: "High DEPTOR expression correlates with poor prognosis in patients with esophageal squamous cell carcinoma." in: OncoTargets and therapy, Vol. 8, pp. 3449-55, (2015) (PubMed).
抗原

DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

别名

Deptor (DEPTOR 产品)

别名

DEP.6 antibody, DEPDC6 antibody, 4731402B04Rik antibody, 9130412E02Rik antibody, D15Ertd336e antibody, D15Ertd597e antibody, Depdc6 antibody, Depdc6-001 antibody, Depdc6-002 antibody, R75183 antibody, mKIAA4200 antibody, RGD1561030 antibody, RAPGEF4 antibody, DEP domain containing MTOR interacting protein antibody, DEP domain containing MTOR-interacting protein antibody, DEPTOR antibody, Deptor antibody

背景

Synonyms: DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6, DEPTOR
Background: DEPTOR (DEP domain containing MTOR-interacting protein), also known as DEP.6 or DEPDC6 (DEP domain-containing protein 6), is a 409 amino acid protein that negatively regulates mTORC1 and mTORC2 pathways. DEPTOR interacts with FRAP via its PDZ domain, and undergoes post-translational phosphorylation. Containing two DEP domains and one PDZ (DHR) domain, DEPTOR is encoded by a gene that maps to human chromosome 8q24.12. Chromosome 8 consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.

基因ID

64798

UniProt

Q8TB45

途径

PI3K-Akt Signaling, RTK signaling, Regulation of Cell Size, BCR Signaling, Warburg Effect