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CD19 抗体 (AA 151-250)

Name: CD19 抗体 (AA 151-250)
SKU: ABIN1387313
Availability: OutOfStock

CD19 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD19 Polyclonal Antibody, PE-Cy5 Conjugated .

FACS

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

ELISA

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD19 Polyclonal Antibody, Unconjugated (ABIN1387313) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-CD19 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C.

IF (p)

Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded fetal mouse brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

7 images

产品编号 ABIN1387313

发货至: 中国

Datasheet as PDF

抗原 See all CD19 抗体

CD19 (CD19 Molecule (CD19))
抗原表位
28

19

16

15

10

7

5

4

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 151-250
适用
292

161

47

30

19

10

7

6

3

3

2

2

2

2

2

2

2

2
人, 小鼠, 大鼠
宿主
172

139

47

4
兔
Compatible Secondaries
克隆类型
231

127

2
多克隆
标记
111

22

21

19

16

8

8

8

7

7

7

7

7

7

7

6

6

6

4

4

4

4

4

4

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This CD19 antibody is un-conjugated
应用范围
262

115

71

62

60

39

22

20

17

14

14

9

5

4

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

人, 小鼠, 大鼠

预测反应

Pig,Guinea Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CD19

亚型

IgG

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anti-CD19 Molecule (CD19) (AA 96-281) antibody
CD19 适用: 人 FACS, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CD19-3116 unconjugated

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CD19 适用: 人 FACS, WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 143-172) antibody
CD19 适用: 人 FACS, WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 160-258) antibody
CD19 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD19 Molecule (CD19) (AA 20-291) antibody
CD19 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6G1 unconjugated

anti-CD19 Molecule (CD19) (AA 96-281) antibody
CD19 适用: 人 FACS, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CD19-3117 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

CD19 (CD19 Molecule (CD19))

别名

CD19 (CD19 产品)

别名

B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

背景

Synonyms: B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19
Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

基因ID

930

UniProt

P15391

途径

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway