FOXP3 抗体 (Forkhead Box P3) (N-Term)

Details for Product anti-FOXP3 Antibody No. ABIN1302424, 供应商: Log in to see
抗原
  • foxp3
  • FOXP3
  • AIID
  • DIETER
  • IPEX
  • PIDX
  • XPID
  • JM2
  • scurfin
  • sf
  • RGD1562112
  • forkhead box P3
  • forkhead box P3a
  • forkhead box P3 L homeolog
  • FOXP3
  • foxp3a
  • foxp3
  • foxp3.L
  • Foxp3
抗原表位
N-Term
80
47
17
14
13
12
11
9
8
8
8
7
7
6
5
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠
414
284
70
44
20
14
9
6
4
3
3
2
1
1
1
1
1
1
宿主
小鼠
230
199
36
34
3
克隆类型 (克隆位点)
单克隆 ()
标记
This FOXP3 antibody is un-conjugated
38
33
24
20
20
19
15
10
8
8
6
6
6
6
5
3
3
3
3
2
2
2
2
应用范围
Flow Cytometry (FACS), Western Blotting (WB)
285
222
186
97
91
80
73
63
28
16
5
5
5
4
3
3
2
1
1
选项
Supplier
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Supplier Product No.
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免疫原 Full-length His-tagged recombinant murine FoxP3
克隆位点 3G3
亚型 IgG1
特异性 The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor, which is the master regulator in the development and function of regulatory T cells.
纯化方法 Purified from ascites by protein-A affinity chromatography.
纯度 > 95 % (by SDS-PAGE)
别名 FOXP3 (FOXP3 Antibody 摘要)
背景 FoxP3 (Forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.
途径 Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
浓度 1 mg/mL
缓冲液 Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
厂商提供的图像
Immunofluorescence (IF) image for anti-Forkhead Box P3 (FOXP3) (N-Term) antibody (ABIN1302424) Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with ant...
背景 Barnes, Powrie: "Regulatory T cells reinforce intestinal homeostasis." in: Immunity, Vol. 31, Issue 3, pp. 401-11, 2009 (PubMed).

Bettini, Vignali: "Regulatory T cells and inhibitory cytokines in autoimmunity." in: Current opinion in immunology, Vol. 21, Issue 6, pp. 612-8, 2009 (PubMed).

Lal, Bromberg: "Epigenetic mechanisms of regulation of Foxp3 expression." in: Blood, Vol. 114, Issue 18, pp. 3727-35, 2009 (PubMed).

Kuhn, Beissert, Krammer: "CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus." in: Archives of dermatological research, Vol. 301, Issue 1, pp. 71-81, 2008 (PubMed).

Elkord: "Novel therapeutic strategies by regulatory T cells in allergy." in: Chemical immunology and allergy, Vol. 94, pp. 150-7, 2008 (PubMed).

有引用在: Law, Hirschkorn, Owen, Biswas, Norris, Lanteri: "The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 75, Issue 12, pp. 1040-50, 2009 (PubMed).

Gavin, Torgerson, Houston, DeRoos, Ho, Stray-Pedersen, Ocheltree, Greenberg, Ochs, Rudensky: "Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 17, pp. 6659-64, 2006 (PubMed).