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SMAD3 抗体 (pSer423, pSer425)

Name: SMAD3 抗体 (pSer423, pSer425)
Brand: antibodies-online
SKU: ABIN129675
Availability: OutOfStock

SMAD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western Blot of Rabbit anti-SMAD3 pS423 pS425 antibody.

affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.

IHC

Western blot using affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates.

Proximity between AcSDKP and FGFR1 inhibits the TGFβ/smad signaling pathway in HMVECs.

AcSDKP suppresses TGFβ/smad signaling and EndMT through the FGFR1/FRS2 pathway.

MAP4K4 deficiency induces TGFβ/smad signaling and EndMT via activation of integrin β1.

7 images

(18 references)

产品编号 ABIN129675

发货至: 中国

Datasheet as PDF

抗原 See all SMAD3 抗体

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
抗原表位
33

28

28

28

20

14

14

9

8

7

6

6

5

5

5

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 417-425, pSer423, pSer425
适用
243

150

138

28

10

9

8

8

7

5

4

3

3

2

1

1

1
人
宿主
234

23
兔
Compatible Secondaries
克隆类型
217

40
多克隆
标记
136

17

14

13

10

7

6

6

6

6

6

6

4

4

4

4

4

1

1

1

1
This SMAD3 antibody is un-conjugated
应用范围
217

116

94

74

59

55

55

53

28

13

10

8

5

4

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

交叉反应

非洲爪蟾, Xenopus tropicalis, 斑马鱼, 大鼠, 小鼠, 猪, 绵羊, 小鸡

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.

亚型

IgG
anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 7F3 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-230) antibody
SMAD3 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) antibody
SMAD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C11 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 3D1 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibody
SMAD3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (C-Term) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pThr179) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
KO Validated SMAD3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Hutchinson, Darling, Nicolaou, Gori, Squair, Cohen, Hill, Sapkota: "Salt-inducible kinases (SIKs) regulate TGFβ-mediated transcriptional and apoptotic responses." in: Cell death & disease, Vol. 11, Issue 1, pp. 49, (2020) (PubMed).

Stappenbeck, Wang, Tang, Zhang, Parhami: "Inhibition of Non-Small Cell Lung Cancer Cells by Oxy210, an Oxysterol-Derivative that Antagonizes TGFβ and Hedgehog Signaling." in: Cells, Vol. 8, Issue 10, (2020) (PubMed).

Feng, Tang, Huang, Sun, You, Xiao, Lv, Xu, Lan: "TGF-β Mediates Renal Fibrosis via the Smad3-Erbb4-IR Long Noncoding RNA Axis." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 26, Issue 1, pp. 148-161, (2019) (PubMed).

Subramanian, Kanzaki, Galloway, Schilling: "Mechanical force regulates tendon extracellular matrix organization and tenocyte morphogenesis through TGFbeta signaling." in: eLife, Vol. 7, (2019) (PubMed).

Gao, Kanasaki, Li, Kitada, Okazaki, Koya: "βklotho is essential for the anti-endothelial mesenchymal transition effects of N-acetyl-seryl-aspartyl-lysyl-proline." in: FEBS open bio, Vol. 9, Issue 5, pp. 1029-1038, (2019) (PubMed).

Tang, Tang, Xu, Li, Deng, Zhang: "Generation of Smurf2 Conditional Knockout Mice." in: International journal of biological sciences, Vol. 14, Issue 5, pp. 542-548, (2018) (PubMed).

Li, Chung, Li, Overstreet, Gagnon, Grouix, Leduc, Laurin, Zhang, Harris: "Fatty acid receptor modulator PBI-4050 inhibits kidney fibrosis and improves glycemic control." in: JCI insight, Vol. 3, Issue 10, (2018) (PubMed).

Li, Shi, Srivastava, Kitada, Nagai, Nitta, Kohno, Kanasaki, Koya: "FGFR1 is critical for the anti-endothelial mesenchymal transition effect of N-acetyl-seryl-aspartyl-lysyl-proline via induction of the MAP4K4 pathway." in: Cell death & disease, Vol. 8, Issue 8, pp. e2965, (2018) (PubMed).

Chung, Overstreet, Li, Wang, Niu, Wang, Fan, Sasaki, Jin, Khodo, Gewin, Zhang, Harris: "TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration." in: JCI insight, Vol. 3, Issue 21, (2018) (PubMed).

Tang, Heller, Meng, Yu, Tang, Zhou, Zhang: "Transforming Growth Factor-β (TGF-β) Directly Activates the JAK1-STAT3 Axis to Induce Hepatic Fibrosis in Coordination with the SMAD Pathway." in: The Journal of biological chemistry, Vol. 292, Issue 10, pp. 4302-4312, (2017) (PubMed).

Subathra, Korrapati, Howell, Arthur, Shayman, Schnellmann, Siskind: "Kidney glycosphingolipids are elevated early in diabetic nephropathy and mediate hypertrophy of mesangial cells." in: American journal of physiology. Renal physiology, Vol. 309, Issue 3, pp. F204-15, (2015) (PubMed).

Luo, Xu, Chen, Warburton, Dong, Qian, Selman, Gauldie, Kolb, Shi: "A novel profibrotic mechanism mediated by TGFβ-stimulated collagen prolyl hydroxylase expression in fibrotic lung mesenchymal cells." in: The Journal of pathology, Vol. 236, Issue 3, pp. 384-94, (2015) (PubMed).

Herhaus, Al-Salihi, Macartney, Weidlich, Sapkota: "OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3." in: Nature communications, Vol. 4, pp. 2519, (2014) (PubMed).

Kawamura, Maeda, Imamura, Setoguchi, Yokouchi, Ishidou, Komiya: "SnoN suppresses maturation of chondrocytes by mediating signal cross-talk between transforming growth factor-β and bone morphogenetic protein pathways." in: The Journal of biological chemistry, Vol. 287, Issue 34, pp. 29101-13, (2012) (PubMed).

Beckham, Tuttle, Tyler: "Reovirus activates transforming growth factor beta and bone morphogenetic protein signaling pathways in the central nervous system that contribute to neuronal survival following infection." in: Journal of virology, Vol. 83, Issue 10, pp. 5035-45, (2009) (PubMed).

Coffman, Coluccio, Planchart, Robertson: "Oral-aboral axis specification in the sea urchin embryo III. Role of mitochondrial redox signaling via H2O2." in: Developmental biology, Vol. 330, Issue 1, pp. 123-30, (2009) (PubMed).

Yamashita, Fatyol, Jin, Wang, Liu, Zhang: "TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta." in: Molecular cell, Vol. 31, Issue 6, pp. 918-24, (2008) (PubMed).

Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, (2003) (PubMed).
抗原

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

别名

SMAD3 (SMAD3 产品)

别名

HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody

背景

This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody

基因ID

4088, 5174513

UniProt

P84022

途径

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy