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PLK1 抗体 (pThr210)

Name: PLK1 抗体 (pThr210)
Brand: antibodies-online
SKU: ABIN129581
Availability: OutOfStock

PLK1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs.

Affinity Purified Plk1 pT210 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue.

IHC

Western blot analysis is shown using Affinity Purified anti-Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead).

Affinity Purified Plk1 pT210 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue.

IHC

4 images

(1 reference)

产品编号 ABIN129581

发货至: 中国

Datasheet as PDF

抗原 See all PLK1 抗体

PLK1 (Polo-Like Kinase 1 (PLK1))
抗原表位
24

17

15

12

10

8

7

6

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 205-214, pThr210
适用
136

50

45

8

8

6

5

5

5

3

3

3

1

1

1

1

1

1
人
宿主
114

21

2

1
兔
Compatible Secondaries
克隆类型
111

27
多克隆
标记
79

7

7

5

4

4

4

4

4

4

3

3

2

2

2

2

2
This PLK1 antibody is un-conjugated
应用范围
108

52

35

28

27

20

19

16

10

7

5

4

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

产品特性

Concentration Definition: by UV absorbance at 280 nm

纯化方法

This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

免疫原

Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.

亚型

IgG

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应用备注

This affinity-purified antibody has been tested for use in ELISA, IHC and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract. This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Adler, Müller, Rached, Dekant, Mally: "Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, (2009) (PubMed).
抗原

PLK1 (Polo-Like Kinase 1 (PLK1))

别名

Plk1 (PLK1 产品)

别名

PLK antibody, STPK13 antibody, plk antibody, PLK-1 antibody, Plx1 antibody, stpk13 antibody, Plk antibody, cb525 antibody, cb636 antibody, ik:tdsubc_2d1 antibody, wu:fb37g11 antibody, wu:fb76g03 antibody, xx:tdsubc_2d1 antibody, polo like kinase 1 antibody, polo like kinase 1 S homeolog antibody, polo-like kinase 1 antibody, polo-like kinase 1 (Drosophila) antibody, Serine/threonine-protein kinase plk-1 antibody, PLK1 antibody, plk1.S antibody, plk1 antibody, Plk1 antibody, plk-1 antibody

背景

Polo-like Kinase pT210 Antibody detects phosphorylated Plk1 protein. Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G₁ or S phase and is associated with the phosphorylation of cyclin B₁. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G₂ and M phases, declines to a nearly undetectable level following mitosis and throughout G₁ phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.
Synonyms: PLK 1 antibody, PLK antibody, polio like kinase antibody, Polo like kinase 1 antibody, Polo-like kinase 1 antibody, Serine/threonine protein kinase 13 antibody

基因ID

5347, 21359873

UniProt

P53350

途径

Cell Division Cycle, M Phase