GFP 抗体 (AA 246) (Alkaline Phosphatase (AP))
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- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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抗原表位
- AA 246
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适用
- Aequorea victoria
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GFP antibody is conjugated to Alkaline Phosphatase (AP)
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应
- recombinant GFP (rGFP), enhanced GFP (eGFP)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
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The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant - 克隆位点
- 9F9-F9
- 亚型
- IgG
- Top Product
- Discover our top product GFP Primary Antibody
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC
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- 应用备注
- Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- 别名
- GFP (GFP 产品)
- 别名
- green fluorescent protein antibody, gfp antibody
- 物质类
- Viral Protein
- 背景
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Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: ALP, Alk Phos, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
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