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CD34 抗体

CD34 适用: 小鼠 FACS, IP 宿主: 大鼠 Monoclonal MEC14-7 unconjugated
产品编号 ABIN119264
发货至: 中国
  • 抗原 See all CD34 抗体
    CD34
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    大鼠
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    单克隆
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    This CD34 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Immunoprecipitation (IP)
    特异性
    This antibody is specific for the CD34 cell surface antigen. It recognises a Neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.
    交叉反应 (详细)
    Species reactivity (tested):Mouse.
    纯化方法
    Affinity Chromatography on Protein G.
    克隆位点
    MEC14-7
    亚型
    IgG2a
    Top Product
    Discover our top product CD34 Primary Antibody
  • 应用备注
    Flow Cytometry: Use 10 μL of 1/10-1/20 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation (2-10 μg/mL). Immunohistochemistry on Frozen and Paraffin Sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    CD34
    别名
    CD34 (CD34 产品)
    别名
    AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody
    背景
    The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. CD34 is a cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
    基因ID
    12490
    NCBI登录号
    NP_598415
    UniProt
    Q64314
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