山羊 anti-Cow IgG (Whole Molecule) Antibody (Biotin) - Preadsorbed
WB, IHC (fro), IHC (p), EIA
Polyclonal
Biotin
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- 抗原 See all IgG products
- IgG
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抗原表位
- Whole Molecule
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适用
- Cow
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 产品特性
- Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
- Bovine IgG whole molecule.
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Rabbit anti-Cow IgG (Fc Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated
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- 应用备注
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 g of Bovine IgG in a standardcapture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
- 浓度
- 2.0 mg/mL
- 缓冲液
- 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 contaning 0.01 % (w/v) Thimerisol as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- Biohazard Informations: This product contains thimerosal which is hazardous.
- 注意事项
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Synonyms: Bovine Immunoglobulin G
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