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MYL12A 抗体 (pSer19, pSer20)

MYL12A 适用: Avian, 哺乳动物 WB, IP, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN117974
发货至: 中国
  • 抗原 See all MYL12A 抗体
    MYL12A (Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A))
    抗原表位
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer19, pSer20
    适用
    • 11
    • 5
    • 5
    Avian, 哺乳动物
    宿主
    • 11
    克隆类型
    • 11
    多克隆
    标记
    • 11
    This MYL12A antibody is un-conjugated
    应用范围
    • 10
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    序列
    K-K-R-P-Q-R-A-T-(pS)-N-V-F-A-M-F-D (aa 12-27 of Human sequence, aa 12-28 of Mouse)
    特异性
    This antibody is specific for the phosphorylated form of myosin light chain. This affinity purified antibody is directed against the regulatory light chain of smooth and nonmuscle myosin. The antibody is phosphospecific and detect monophosphorylated and diphosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS20 of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1 % by ELISA. Cross reactivity with myosin light chain from other species has not been determined, however, the sequence of the immunogen is identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
    交叉反应 (详细)
    Species reactivity (tested):Human.
    纯化方法
    Immunoaffinity Chromatography.
    免疫原
    Human Myosin Light Chain phospho peptide corresponding to a region of the Human smooth/non-muscle form of Myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).
    Top Product
    Discover our top product MYL12A Primary Antibody
  • 应用备注
    This phospho specific polyclonal antibody was tested by ELISA, Immunohistochemistryand Immunoblotting and was found to be reactive with unstimulated and stimulatedcardiac myocytes and the phosphorylated form of the immunizing peptide and minimallyreactive with the non-phosphorylated form of the immunizing peptide.
    限制
    仅限研究用
  • 浓度
    0.92 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 2-8 °C. DO NOT FREEZE!
  • Kaufmann, Martinka, Moede, Sendeski, Steege, Fähling, Hultström, Gaestel, Moraes-Silva, Nikitina, Liu, Zavaritskaya, Patzak: "Noradrenaline enhances angiotensin II responses via p38 MAPK activation after hypoxia/re-oxygenation in renal interlobar arteries." in: Acta physiologica (Oxford, England), Vol. 213, Issue 4, pp. 920-32, (2015) (PubMed).

    Young, Kretchmer, Ondeck, Zambon, Engler: "Mechanosensitive kinases regulate stiffness-induced cardiomyocyte maturation." in: Scientific reports, Vol. 4, pp. 6425, (2015) (PubMed).

    Gao, Peleli, Zollbrecht, Patzak, Persson, Carlström: "Adenosine A1 receptor-dependent and independent pathways in modulating renal vascular responses to angiotensin II." in: Acta physiologica (Oxford, England), Vol. 213, Issue 1, pp. 268-76, (2014) (PubMed).

    Schott, Grosskinsky, Brenner, Kraiczy, Wallich: "Molecular characterization of the interaction of Borrelia parkeri and Borrelia turicatae with human complement regulators." in: Infection and immunity, Vol. 78, Issue 5, pp. 2199-208, (2010) (PubMed).

    Patzak, Lai, Fähling, Sendeski, Martinka, Persson, Persson: "Adenosine enhances long term the contractile response to angiotensin II in afferent arterioles." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 293, Issue 6, pp. R2232-42, (2008) (PubMed).

    Lai, Martinka, Fähling, Mrowka, Steege, Gericke, Sendeski, Persson, Persson, Patzak: "Adenosine restores angiotensin II-induced contractions by receptor-independent enhancement of calcium sensitivity in renal arterioles." in: Circulation research, Vol. 99, Issue 10, pp. 1117-24, (2006) (PubMed).

  • 抗原
    MYL12A (Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A))
    别名
    MYL12A (MYL12A 产品)
    别名
    MLCB antibody, MRCL3 antibody, MRLC3 antibody, MYL2B antibody, RGD1309537 antibody, Rlc-a antibody, myosin light chain 12A antibody, MYL12A antibody, Myl12a antibody
    背景
    Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.Synonyms: MLC-2B, MLCB, MRLC3, Myosin RLC, Myosin regulatory light chain 12A, Myosin regulatory light chain 2 nonsarcomeric, Myosin regulatory light chain MRLC3, RLC
    基因ID
    10627
    NCBI登录号
    NP_006462
    UniProt
    P19105
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