Phospholipase C gamma 1 抗体 (pTyr783, Tyr783) (Alexa Fluor 647)
(1 reference)-
- 抗原 See all Phospholipase C gamma 1 (PLCG1) 抗体
- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
-
抗原表位
- pTyr783, Tyr783
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Phospholipase C gamma 1 antibody is conjugated to Alexa Fluor 647
-
应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated peptide including the tyrosine 783 of human PLCgamma1
- 克隆位点
- 27-PLC
- 亚型
- IgG1
- Top Product
- Discover our top product PLCG1 Primary Antibody
-
anti-phospholipase C, gamma 1 (PLCG1) (AA 1192-1291) antibody
PLCG1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 1091-1290) antibodyPLCG1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (pTyr771) antibodyPLCG1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 1192-1291) antibodyPLCG1 适用: 人, 小鼠 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 3H1C10 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 1192-1291) antibodyPLCG1 适用: 人, 大鼠, 猴 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 3C2D11 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 39-181) antibodyPLCG1 适用: 人, 大鼠 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F3D11 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (pTyr783) antibodyPLCG1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 39-181) antibodyPLCG1 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F3E10 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (pTyr783) antibodyPLCG1 适用: 小鼠, 大鼠 WB, ELISA, IF (p), IHC (p), FACS, IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (Tyr771) antibodyPLCG1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
- 样本量
- 20 μL
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
-
-
: "Phosphorylation of phospholipase Cgamma1 on tyrosine residue 783 by platelet-derived growth factor regulates reorganization of the cytoskeleton." in: Experimental cell research, Vol. 243, Issue 1, pp. 113-22, (1998) (PubMed).
-
: "Phosphorylation of phospholipase Cgamma1 on tyrosine residue 783 by platelet-derived growth factor regulates reorganization of the cytoskeleton." in: Experimental cell research, Vol. 243, Issue 1, pp. 113-22, (1998) (PubMed).
-
- 抗原
- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
- 别名
- PLC-gamma 1 (PLCG1 产品)
- 背景
- The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCg is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCg1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLCg is essential for growth factor-induced cell motility and mitogenesis. PLCg1-null mice exhibit retarded embryonic growth and lethality in midgestation. In addition, PDGF stimulation leads to phosphorylation of PLCg1 at Tyr 783 and activation of hydrolyzing activity. Overexpression of PLCg is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus, regulation of PLCg activity by growth factors is involved in cell growth and transformation. The 27/PLC antibody recognizes PLCg1 phosphorylated at Y783. The fluorochrome-conjugated formats have been evaluated using a human model system. However, the unconjugated form of this antibody (Cat. no. 612464) has been shown to react with human, mouse, and rat cell lysates by western blot.
- 途径
- RTK signaling, WNT signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling
-
