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STAT1 抗体 (pTyr701) (Alexa Fluor 647)

STAT1 适用: 人, 小鼠 ICS 宿主: 小鼠 Monoclonal 4a Alexa Fluor 647
产品编号 ABIN1177190
发货至: 中国
  • 抗原 See all STAT1 抗体
    STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
    抗原表位
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    pTyr701
    适用
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    人, 小鼠
    宿主
    • 188
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    小鼠
    克隆类型
    • 165
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    单克隆
    标记
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    This STAT1 antibody is conjugated to Alexa Fluor 647
    应用范围
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    Intracellular Staining (ICS)
    品牌
    BD Phosflow™
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Phosphorylated Human Stat1 Peptide
    克隆位点
    4a
    亚型
    IgG2a
    Top Product
    Discover our top product STAT1 Primary Antibody
  • 样本量
    20 μL
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
  • Tanaka, Saito, Kunisawa, Kurashima, Wake, Suzuki, Shultz, Kiyono, Ishikawa: "Development of mature and functional human myeloid subsets in hematopoietic stem cell-engrafted NOD/SCID/IL2rγKO mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 12, pp. 6145-55, (2012) (PubMed).

    Suni, Maino: "Flow cytometric analysis of cell signaling proteins." in: Methods in molecular biology (Clifton, N.J.), Vol. 717, pp. 155-69, (2011) (PubMed).

    Perez, Mitchell, Campos, Gao, Li, Nolan: "Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry." in: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

    Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, (2000) (PubMed).

    Darnell: "STATs and gene regulation." in: Science (New York, N.Y.), Vol. 277, Issue 5332, pp. 1630-5, (1997) (PubMed).

    Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, (1993) (PubMed).

  • 抗原
    STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))
    别名
    Stat1 (STAT1 产品)
    别名
    CANDF7 antibody, ISGF-3 antibody, STAT91 antibody, 2010005J02Rik antibody, AA408197 antibody, XStat1 antibody, STAT4 antibody, DD6G4-4 antibody, STAT antibody, STAT1 antibody, dZ182H3.3 antibody, si:by134g18.2 antibody, si:by51f19.1 antibody, si:dz182h3.3 antibody, si:dz199m19.1 antibody, si:dz73o4.2 antibody, stat1 antibody, signal transducer and activator of transcription 1 antibody, signal transducer and activator of transcription 1 L homeolog antibody, signal transducer and activator of transcription 1, 91kDa antibody, signal transducer and activator of transcription 4 antibody, signal transducer and activator of transcription 1a antibody, STAT1 antibody, Stat1 antibody, stat1.L antibody, stat4 antibody, stat1a antibody
    背景
    Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84-kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). The 4a monoclonal antibody recognizes the phosphorylated Y701 in Stat1alpha and Stat1beta.
    途径
    JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events
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