ELK1 抗体 (pSer383) (Alexa Fluor 647)
(5 references)-
- 抗原 See all ELK1 抗体
- ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))
-
抗原表位
- pSer383
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This ELK1 antibody is conjugated to Alexa Fluor 647
-
应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Human Elk-1 Peptide
- 克隆位点
- M21-1721
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product ELK1 Primary Antibody
-
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibody
ELK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer389) antibodyELK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (C-Term) antibodyELK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) antibodyELK1 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 3H6D12 unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibodyELK1 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (AA 247-403) antibodyELK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pThr417) antibodyELK1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer389) antibodyELK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibodyELK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (AA 67-166) antibodyELK1 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 2G6 unconjugated
-
- 应用备注
- This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used.
- 样本量
- 20 μL
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
-
-
: "Docking sites on mitogen-activated protein kinase (MAPK) kinases, MAPK phosphatases and the Elk-1 transcription factor compete for MAPK binding and are crucial for enzymic activity." in: The Biochemical journal, Vol. 370, Issue Pt 3, pp. 1077-85, (2003) (PubMed).
: "Mapping of synergistic components of weakly interacting protein-protein motifs using arrays of paired peptides." in: The Journal of biological chemistry, Vol. 278, Issue 17, pp. 15162-7, (2003) (PubMed).
: "Stimulation of Elk1 transcriptional activity by mitogen-activated protein kinases is negatively regulated by protein phosphatase 2B (calcineurin)." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 15173-80, (1999) (PubMed).
: "The calcium/calmodulin-dependent protein phosphatase calcineurin is the major Elk-1 phosphatase." in: The Journal of biological chemistry, Vol. 272, Issue 47, pp. 29415-8, (1997) (PubMed).
: "Induction of c-fos expression through JNK-mediated TCF/Elk-1 phosphorylation." in: The EMBO journal, Vol. 14, Issue 23, pp. 5957-64, (1996) (PubMed).
-
: "Docking sites on mitogen-activated protein kinase (MAPK) kinases, MAPK phosphatases and the Elk-1 transcription factor compete for MAPK binding and are crucial for enzymic activity." in: The Biochemical journal, Vol. 370, Issue Pt 3, pp. 1077-85, (2003) (PubMed).
-
- 抗原
- ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))
- 别名
- Elk-1 (ELK1 产品)
- 别名
- Elk-1 antibody, Xelk antibody, Xelk-1 antibody, elk-1 antibody, elk1 antibody, ELK1, ETS transcription factor antibody, ELK1, member of ETS oncogene family antibody, ETS domain-containing protein Elk-1 antibody, ELK1, member of ETS oncogene family S homeolog antibody, ELK1 antibody, Elk1 antibody, Tsp_02690 antibody, elk1 antibody, elk1.S antibody
- 背景
-
Elk-1 is a 62-kDa member of the Ets oncogene family of transcription factors and a member of the subfamily of ternary complex factors (TCF). Ets proteins mediate a variety of gene activities in response to serum and growth factors. Proteins in the TCF subfamily form a ternary complex by binding to the Serum Response Element (SRE) in conjunction with a dimer of Serum Response Factors (SRF). Elk-1 is phosphorylated by mitogen-activated protein (MAP) kinase pathways in vivo at a cluster of S/T motifs at its carboxy-terminus. Phosphorylation at these sites, particularly at serine 383 (S383), is critical for Elk-1 transcriptional activation. Studies have shown that Elk-1 is a direct target of activated MAP kinase, and that it is also a target of the stress-activated kinase SAPK/JNK. The M21-1721 monoclonal antibody recognizes the phosphorylated S383 of activated Elk-1. The orthologous phosphorylation sites of mouse and rat Elk-1 are S384 and S382, respectively.
Synonyms: ELK1 - 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
-
