电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

PKC alpha 抗体 (AA 270-427) (Alexa Fluor 488)

PKCa 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Frog ICS 宿主: 小鼠 Monoclonal 3-PKCalpha Alexa Fluor 488
产品编号 ABIN1176927
发货至: 中国
  • 抗原 See all PKC alpha (PKCa) 抗体
    PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
    抗原表位
    • 24
    • 20
    • 18
    • 16
    • 12
    • 10
    • 8
    • 7
    • 7
    • 6
    • 6
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 270-427
    适用
    • 183
    • 90
    • 68
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, 犬, 小鸡, Frog
    宿主
    • 179
    • 12
    • 1
    小鼠
    克隆类型
    • 145
    • 46
    单克隆
    标记
    • 96
    • 16
    • 14
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This PKC alpha antibody is conjugated to Alexa Fluor 488
    应用范围
    • 133
    • 76
    • 58
    • 33
    • 22
    • 20
    • 17
    • 15
    • 14
    • 8
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Intracellular Staining (ICS)
    品牌
    BD Phosflow™
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Human PKCalpha aa. 270-427 Recombinant Protein
    克隆位点
    3-PKCalpha
    亚型
    IgG2b kappa
    Top Product
    Discover our top product PKCa Primary Antibody
  • 应用备注
    Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
    样本量
    20 μL
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Braz, Gregory, Pathak, Zhao, Sahin, Klevitsky, Kimball, Lorenz, Nairn, Liggett, Bodi, Wang, Schwartz, Lakatta, DePaoli-Roach, Robbins, Hewett, Bibb, Westfall, Kranias, Molkentin: "PKC-alpha regulates cardiac contractility and propensity toward heart failure." in: Nature medicine, Vol. 10, Issue 3, pp. 248-54, (2004) (PubMed).

    Parker, Murray-Rust: "PKC at a glance." in: Journal of cell science, Vol. 117, Issue Pt 2, pp. 131-2, (2003) (PubMed).

    Zhang, Bontrager, Hemler: "Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25005-13, (2001) (PubMed).

    Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, (1988) (PubMed).

  • 抗原
    PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
    别名
    PKCa (PKCa 产品)
    别名
    pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody
    背景
    The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. Conventional PKC (cPKC) subfamily members (a, b, and gamma isoforms) consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the the C2-like domains of novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) are Ca2+-independent. The atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCalpha regulates a wide variety of functions such as cellular growth, apoptosis, cardiomyocyte function, and brain cognitive functions. The 3/PKCalpha monoclonal antibody recognizes PKCalpha, regardless of phosphorylation status, and has been reported to crossreact with PKCbeta.
    Synonyms: Protein Kinase C alpha, PKC-alpha, PKC-A, PKCA, PRKCA
    途径
    WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
You are here:
客服