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CD3 epsilon 抗体

CD3E 适用: 小鼠 FACS, WB, IP, IHC (fro), BR, CyTox, FM 宿主: Armenian Hamster Monoclonal 145-2C11 unconjugated
产品编号 ABIN1176869
发货至: 中国
  • 抗原 See all CD3 epsilon (CD3E) 抗体
    CD3 epsilon (CD3E)
    适用
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    小鼠
    宿主
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    Armenian Hamster
    克隆类型
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    单克隆
    标记
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    This CD3 epsilon antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Cytotoxicity Test (CyTox), Fluorescence Microscopy (FM)
    品牌
    BD Pharmingen™
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    过滤
    0.2 μm filtered
    内毒素水平
    Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
    免疫原
    H-2Kb specific cytotoxic T lymphocyte clone BM10-37
    克隆位点
    145-2C11
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD3E Primary Antibody
  • 限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液
    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
    储存液
    Azide free
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • Chai, Lechler: "Immobilized anti-CD3 mAb induces anergy in murine naive and memory CD4+ T cells in vitro." in: International immunology, Vol. 9, Issue 7, pp. 935-44, (1997) (PubMed).

    Nakano, Yamazaki, Miyatake, Nozaki, Kikuchi, Saito: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, (1996) (PubMed).

    Wagner, Hagman, Linsley, Hodsdon, Freed, Newell: "Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1631-8, (1996) (PubMed).

    Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, (1995) (PubMed).

    Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, (1994) (PubMed).

    Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." in: International immunology, Vol. 6, Issue 7, pp. 995-1001, (1994) (PubMed).

    Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, (1991) (PubMed).

    Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, (1989) (PubMed).

    Portoles, Rojo, Golby, Bonneville, Gromkowski, Greenbaum, Janeway, Murphy, Bottomly: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, (1989) (PubMed).

    Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, (1987) (PubMed).

  • 抗原
    CD3 epsilon (CD3E)
    别名
    CD3e (CD3E 产品)
    别名
    cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
    背景
    The 145-2C11 antibody reacts with the 25-kDa epsilon chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3epsilon and anti-alphabeta TCR mAbs to detect the T-cell receptor on immature thymocytes.
    Synonyms: CD3epsilon chain
    途径
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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