It has been selected for its ability to recognize IgG in immunohistochemical staining and Western blotting.
纯化方法
Affinity Chromatography
免疫原
Native Protein IgG Immunoglobulin G (IgG) is one of the most abundant proteins in serum and the major components of the immune system. IgG is important for our defence against microorganisms and the molecules, which are produced by B lymphocytes as a part of our adaptive immune response. By binding many kinds of pathogen—representing viruses, bacteria, and fungi—IgG protects the body from infection. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. The antibody is a rabbit polyclonal antibody raised against IgG.
Optimal working dilutions should be determined experimentally by the investigator.
限制
仅限研究用
状态
Lyophilized
浓度
200 µg/mL
缓冲液
Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项
Avoid repeated freeze/thaw cycles
储存条件
4 °C
储存方法
Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.