F4/80 抗体
(7 references)-
- 抗原 See all F4/80 (EMR1) 抗体
- F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This F4/80 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Immunoelectron Microscopy (IEM)
- 特异性
- This antibody recognises the F4/80 antigen, a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with Human EMR1. Clone CI:A31 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5). We recommend the use of BM4008LE for this purpose.
- 交叉反应 （详细）
- Species reactivity (tested):Mouse.
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
- 克隆位点
- Cl:A3-1
- 亚型
- IgG2b
- Top Product
- Discover our top product EMR1 Primary Antibody
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anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 732-747) antibody
EMR1 适用: 小鼠, 人, 大鼠 FACS, WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 21-290) antibodyEMR1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (C-Term) antibodyEMR1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 1-201) antibodyEMR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (C-Term) antibodyEMR1 适用: 小鼠, 人, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) antibody (PE)EMR1 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal BM8-1 PE
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 21-290) antibodyEMR1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 1-821) antibodyEMR1 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) antibodyEMR1 适用: 小鼠 FACS, WB, IHC (p) 宿主: 大鼠 Monoclonal BM8 unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) antibody (FITC)EMR1 适用: 小鼠 FACS, WB, IHC (p) 宿主: 大鼠 Monoclonal BM8 FITC
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- 应用备注
- RIA. Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Embedded and Resin Sections. Thisproduct requires pre-treatment of paraffin sections (Proteinase K is recommended fortissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixedfor more than 24 hours).
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "Piceatannol is superior to resveratrol in promoting neural stem cell differentiation into astrocytes." in: Food & function, Vol. 7, Issue 10, pp. 4432-4441, (2018) (PubMed).
: "Claudin-3 and Clara cell 10 kDa protein as early signals of cigarette smoke-induced epithelial injury along alveolar ducts." in: Toxicologic pathology, Vol. 40, Issue 8, pp. 1169-87, (2012) (PubMed).
: "Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 2, pp. 808-17, (2012) (PubMed).
: "Tricyclic indole and dihydroindole derivatives as new inhibitors of soluble guanylate cyclase." in: Bioorganic & medicinal chemistry letters, Vol. 19, Issue 16, pp. 4810-3, (2009) (PubMed).
: "Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis." in: Blood, Vol. 113, Issue 19, pp. 4810-8, (2009) (PubMed).
: "Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5728-37, (2007) (PubMed).
: "Monocytes/macrophages cooperate with progenitor cells during neovascularization and tissue repair: conversion of cell columns into fibrovascular bundles." in: The American journal of pathology, Vol. 168, Issue 2, pp. 529-41, (2006) (PubMed).
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: "Piceatannol is superior to resveratrol in promoting neural stem cell differentiation into astrocytes." in: Food & function, Vol. 7, Issue 10, pp. 4432-4441, (2018) (PubMed).
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- 抗原
- F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
- 别名
- Macrophage F4/80 Antigen (EMR1 产品)
- 别名
- EMR1 antibody, TM7LN3 antibody, DD7A5-7 antibody, EGF-TM7 antibody, F4/80 antibody, Gpf480 antibody, Ly71 antibody, adhesion G protein-coupled receptor E1 antibody, ADGRE1 antibody, LOC100424335 antibody, Adgre1 antibody
- 背景
- F4/80 antigen is a 160 kD glycoprotein expressed by most murine macrophages. Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymphnode. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
- 基因ID
- 13733
- NCBI登录号
- NP_034260
- UniProt
- Q61549
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