F4/80 抗体 (Biotin)
(3 references)-
- 抗原 See all F4/80 (EMR1) 抗体
- F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
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适用
- 小鼠, 人
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This F4/80 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody is useful for the detection of major subpopulations of resident tissue macrophages. It is also suitable for the detection of blood monocytes and other monocytic cells isolated from different sources by FACS. This clone is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas and has been shown to be a unique histological marker of the progression from peri-insulitis to b-cell destruction and diabetes in a mouse diabetes model. Antigen Distribution Isolated Cells: expressed in vitro on over 80 % of M-CSF stimulated bone marrow derived macrophages, after a few days of culture. It is absent from granulocytes, lymphocytes and thrombocytes. Tissue Sections: The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. In complete Freund's adjuvant induced granulomas the antigen is expressed by inflammatory macrophages, but is absent from epitheloid cells.
- 交叉反应 （详细）
- Species reactivity (tested):Mouse.Also stains Human Heart Macrophages.
- 纯化方法
- Affinity Chromatography
- 免疫原
- Cultured Macrophages. Remarks: The antigen/epitope is a 125 kDa extracellular membrane protein sensitive to 2-Mercaptoetanol.
- 克隆位点
- BM8
- 亚型
- IgG2a
- Top Product
- Discover our top product EMR1 Primary Antibody
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anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 732-747) antibody
EMR1 适用: 小鼠, 人, 大鼠 FACS, WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 21-290) antibodyEMR1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (C-Term) antibodyEMR1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 1-201) antibodyEMR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (C-Term) antibodyEMR1 适用: 小鼠, 人, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) antibody (PE)EMR1 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal BM8-1 PE
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 21-290) antibodyEMR1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) (AA 1-821) antibodyEMR1 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) antibodyEMR1 适用: 小鼠 FACS, WB, IHC (p) 宿主: 大鼠 Monoclonal BM8 unconjugated
anti-Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1) antibody (FITC)EMR1 适用: 小鼠 FACS, WB, IHC (p) 宿主: 大鼠 Monoclonal BM8 FITC
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- 应用备注
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Immunohistochemistry on Frozen Sections: 1.0 μg/mL (1/200). Immunohistochemistry on Paraffin Sections: not tested, but work well with the purifiedantibody using Proteinase K pretreatment. Recommended Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS, pH 7.2, None, 5 mg/mL BSA
- 储存液
- Without preservative
- 注意事项
- Do Not Use Sodium Azide as Preservative.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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: "Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 2, pp. 733-8, (2011) (PubMed).
: "Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5728-37, (2007) (PubMed).
: "Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 24, Issue 12, pp. 2307-12, (2004) (PubMed).
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: "Targeting membrane heat-shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 2, pp. 733-8, (2011) (PubMed).
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- 抗原
- F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))
- 别名
- Macrophage F4/80 Antigen (EMR1 产品)
- 别名
- EMR1 antibody, TM7LN3 antibody, DD7A5-7 antibody, EGF-TM7 antibody, F4/80 antibody, Gpf480 antibody, Ly71 antibody, adhesion G protein-coupled receptor E1 antibody, ADGRE1 antibody, LOC100424335 antibody, Adgre1 antibody
- 背景
- F4/80 protein binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. F4/80 is not expressed by lymphocytes or polymorphonuclear cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
- 基因ID
- 13733
- NCBI登录号
- NP_034260
- UniProt
- Q61549
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