(1 reference)-
- 抗原 See all CD11c (ITGAX) 抗体
- CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD11c antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD11c APC, is recommended for use in flow cytometry for identification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen.
- 克隆位点
- BU-15
- 亚型
- IgG1
- Top Product
- Discover our top product ITGAX Primary Antibody
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anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) antibody
ITGAX 适用: 人, 小鼠, 大鼠 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) antibody (FITC)ITGAX 适用: 人, 猴 FACS 宿主: 小鼠 Monoclonal BU15 FITC
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 1119-1145) antibodyITGAX 适用: 人 FACS, IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 100-350) antibodyITGAX 适用: 人 IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) antibodyITGAX 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) antibodyITGAX 适用: 人, 猴 FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal BU15 unconjugated
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 102-279) antibodyITGAX 适用: 人 FACS, IHC, WB, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 6G2D1 unconjugated
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 1119-1145), (C-Term) antibodyITGAX 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB21771 unconjugated
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (AA 637-827) antibodyITGAX 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal ITGAX-1242 unconjugated
anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) (N-Term) antibodyITGAX 适用: 人, 小鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Allophycocyanin (Febico, Far East Bio-Tech Co.).
- 样品收集
- 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10 P6 P cells). 2. Add 20 µl of CD11c APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4 °C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis. UTH U
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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: "Incidence of phenotypic aberrations in a series of 467 patients with B chronic lymphoproliferative disorders: basis for the design of specific four-color stainings to be used for minimal residual disease investigation." in: Leukemia, Vol. 16, Issue 8, pp. 1460-9, (2002) (PubMed).
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: "Incidence of phenotypic aberrations in a series of 467 patients with B chronic lymphoproliferative disorders: basis for the design of specific four-color stainings to be used for minimal residual disease investigation." in: Leukemia, Vol. 16, Issue 8, pp. 1460-9, (2002) (PubMed).
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- 抗原
- CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))
- 别名
- CD11c (ITGAX 产品)
- 别名
- CD11C antibody, SLEB6 antibody, RGD1561123 antibody, AI449405 antibody, Cd11c antibody, Cr4 antibody, N418 antibody, integrin subunit alpha X antibody, integrin alpha X antibody, ITGAX antibody, Itgax antibody
- 背景
- This antibody reacts with the X chain of the CD11c/CD18 integrin heterodimer expressed on monocytes and weakly on granulocytes. The antibody stains macrophages in most types of tissues. flow cytometry the antibody labels a T-cell subset and weakly a B-cell subset.
- 途径
- Complement System, Activated T Cell Proliferation, Integrin Complex
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