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Cytokeratin 19 抗体

KRT19 适用: 人, 大鼠, Cow, 小鸡, Amphibian WB, IF, IHC (fro), EIA 宿主: 小鼠 Monoclonal KS19-2(Z105-6) unconjugated
产品编号 ABIN111167
发货至: 中国
  • 抗原 See all Cytokeratin 19 (KRT19) 抗体
    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
    适用
    • 261
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    人, 大鼠, Cow, 小鸡, Amphibian
    宿主
    • 139
    • 131
    • 3
    • 2
    • 1
    小鼠
    克隆类型
    • 144
    • 132
    单克隆
    标记
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    This Cytokeratin 19 antibody is un-conjugated
    应用范围
    • 192
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    • 40
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    序列
    VRADSERQNQ EYQRLMD Remarks: The epitope has been localized on amino acids 352-368 of the alpha-helical fragment.
    特异性
    This antibody represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular Ca.! Recognizes a Mr 40,000 polypeptide (Cytokeratin 19) of Human glandular epithelia. Positive on the following cell lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12. Tumors Specifically Detected: All tested adenocarcinoma, cholangio ca. of liver, renal cell ca., transitional cell ca. of the bladder, ovary ca., squamous cell ca. of cervix, bronchus and lung (intermediate type), mesothelioma, carcinoid tumor of bronchus, breast ca., thymoma.
    交叉反应 (详细)
    Species reactivity (tested):Human, Rat, Fish, Bovine.
    纯化方法
    Affinity Chromatography on Protein A .
    免疫原
    Keratin K19 of Mr 40000 from cultured Human MCF-7 cells
    克隆位点
    KS19-2(Z105-6)
    亚型
    IgG2b
    Top Product
    Discover our top product KRT19 Primary Antibody
  • 应用备注
    ELISA. Western blot. Cytological Material. Immunohistochemistry on Frozen Sections: Dilution Buffer: Dilute 1/10 with PBS, pH 7.4, for use in Immunohistochemistry. Incubation Time: 1 hour at room temperature.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with 1.0 mL distilled water.
    缓冲液
    PBS pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. This reagent is not sterile, but can be sterilized by filtration if necessary. To minimize loss of volume during filtration, dilute to the final working concentration in the appropriate medium before filtration and filter through a 0.45µ Millipore filter (or equivalent).
    储存条件
    -20 °C
    储存方法
    Store at -20 °C or below before reconstitution. Reconstitute with 0.5 ml of cold distilled water. After reconstitution, aliquot and freeze unused portions in volumes appropriate for single usage.
  • 抗原
    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
    别名
    Cytokeratin 19 (KRT19 产品)
    别名
    CK19 antibody, K19 antibody, K1CS antibody, AI663979 antibody, EndoC antibody, Krt-1.19 antibody, Krt1-19 antibody, Ka19 antibody, k19 antibody, ck19 antibody, k1cs antibody, krt9 antibody, krt15 antibody, MGC76282 antibody, GK-19 antibody, MGC83069 antibody, KRT19 antibody, keratin 19 antibody, keratin 19 L homeolog antibody, keratin, type I cytoskeletal 19 antibody, KRT19 antibody, Krt19 antibody, krt19 antibody, krt19.L antibody, LOC100344434 antibody, LOC101117946 antibody
    背景
    Cytokeratin 19 is a type I acidic subfamily of intermediate filament protein that is expressed in stratified and simple type epithelia. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19
    基因ID
    3880
    NCBI登录号
    NP_002267
    UniProt
    P08727
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