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His Tag 抗体

WB, IP, IF 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
产品编号 ABIN1105141
发货至: 中国
  • 抗原 See all His Tag 抗体
    His Tag
    适用
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    宿主
    • 93
    • 56
    • 12
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    • 1
    小鼠
    克隆类型
    • 106
    • 69
    • 10
    单克隆
    标记
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    This His Tag antibody is un-conjugated
    应用范围
    • 153
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    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
    序列
    HHHHHH
    纯化方法
    Affinity Chromatography.
    免疫原
    Synthetic peptide containing the His epitope 6x His (H-H-H-H-H-H).
    克隆位点
    HIS-H8
    亚型
    IgG2b
    Top Product
    Discover our top product His Tag Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store the antibody (in aliquots) at -20 °C.
  • 抗原
    His Tag
    别名
    6xHistidine Epitope Tag (His Tag 产品)
    物质类
    Tag
    背景
    Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
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