V5 Epitope Tag 抗体 (AA 95-108)
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- 抗原 See all V5 Epitope Tag products
- V5 Epitope Tag
- 抗原表位
- AA 95-108
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This V5 Epitope Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 序列
- GKPIPNPLLG LDST
- 特异性
- This affinity-purified antibody is directed against V5 motif and is useful in determining its presence in various assays. This polyclonal anti-V5-tag antibody detects over-expressed proteins containing the V5 epitope tag. To date this antibody has reacted with all V5 tagged proteins tested so far. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. The antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to either the carboxy- terminal end of targeted proteins in transfected or transformed cells. Although not yet tested, expect reactivity with recombinant proteins prepared with the V5-epitope tag fused to the amino terminal end as well.
- 纯化方法
- Affinity purified
- 过滤
- Sterile filtered
- 免疫原
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This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.
Type of Immunogen: Synthetic peptide - 亚型
- IgG
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anti-V5 Epitope Tag antibody
WB 宿主: 山羊 Polyclonal unconjugated
anti-V5 Epitope Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-V5 Epitope Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 8G5 unconjugated
anti-V5 Epitope Tag antibody (HRP)WB, ELISA 宿主: 兔 Polyclonal HRP
anti-V5 Epitope Tag antibodyWB, ELISA, IP, IHC (fro), RIA, IHC 宿主: 小鼠 Monoclonal SV5-Pk1 unconjugated
anti-V5 Epitope Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-V5 Epitope Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
anti-V5 Epitope Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-V5 Epitope Tag antibody适用: Paramyxovirus WB, ELISA, IP, IF, IHC (fro), RIA 宿主: 小鼠 Monoclonal SV5-P-K unconjugated Recombinant Antibody
anti-V5 Epitope Tag antibodyWB, ELISA, IHC 宿主: 小鼠 Monoclonal E10 unconjugated
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- 应用备注
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Approved: ELISA, IHC (1:500 - 1:3000), WB (1:5000 - 1:10000)
Usage: Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Aliquot to Avoid freeze/thaw cycles. Store undiluted.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- V5 Epitope Tag
- 别名
- V5 Tag (V5 Epitope Tag 产品)
- 物质类
- Tag
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