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RFP 抗体

RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated
产品编号 ABIN1103975
发货至: 中国
  • 抗原 See all RFP 抗体
    RFP (Red Fluorescent Protein (RFP))
    适用
    Discosoma
    宿主
    • 23
    • 13
    • 9
    • 5
    • 3
    小鸡
    克隆类型
    • 30
    • 23
    多克隆
    标记
    • 19
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFP antibody is un-conjugated
    应用范围
    • 27
    • 21
    • 15
    • 12
    • 10
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    原理
    Anti-RFP / Red Fluorescent Protein Antibody
    特异性
    Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.
    纯化方法
    Purified
    过滤
    Sterile filtered
    免疫原
    The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

    Type of Immunogen: Fusion protein
    亚型
    IgG
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  • 应用备注
    Approved: ELISA, WB (1:1000 - 1:3000)

    Usage: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid freeze/thaw cycles. Store undiluted.
    储存条件
    -20 °C
    储存方法
    Store at -20°C. Store undiluted.
  • 抗原
    RFP (Red Fluorescent Protein (RFP))
    别名
    RFP (RFP 产品)
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