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VEGFA 抗体 (AA 27-146)

Name: VEGFA 抗体 (AA 27-146)
SKU: ABIN1078647
Availability: OutOfStock

VEGFA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) antibody (ABIN1078647)

Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue

IHC

2 images

(1 reference)

产品编号 ABIN1078647

发货至: 中国

Datasheet as PDF

抗原 See all VEGFA 抗体

VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
抗原表位
19

15

15

15

15

15

10

7

7

6

6

6

6

5

5

5

4

4

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 27-146
适用
155

126

98

24

20

19

19

16

12

7

5

5

4

3

2

2

2

1

1

1
小鼠
宿主
204

53

13

1
兔
Compatible Secondaries
克隆类型
215

55

1
多克隆
标记
122

41

25

12

5

5

5

5

5

5

5

5

5

5

5

4

3

1

1

1

1

1

1

1

1

1
This VEGFA antibody is un-conjugated
应用范围
206

93

65

65

51

32

27

22

22

16

15

12

10

9

8

7

3

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

原理

Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)

序列

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- APTT EGEQKSHEVI KFMDVYQRSY CRPIETLVDI FQEYPDEIEY IFKPSCVPLM RCAGCCNDEA LECVPTSESN ITMQIMRIKP HQSQHIGEMS FLQHSRCECR PKKDRTKPEK KSVRGKGKGQ KRKRKKSRFK SWSVHCEPCS ERRKHLFVQD PQTCKCSCKN

特异性

The antibody is a rabbit polyclonal antibody raised against VEGFA. It has been selected for its ability to recognize VEGFA in immunohistochemical staining and western blotting.

交叉反应

人, 大鼠

纯化方法

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

免疫原

Recombinant Vascular Endothelial Growth Factor A (VEGFA) corresdonding to Ala27~Arg146 with N-terminal His Tag

亚型

IgG

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VEGFA 适用: 人 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated

应用备注

Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.

说明

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freeze/thaw cycles

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

有效期

24 months
Thelin, Frostell, Mulder, Mitsios, Damberg, Aski, Risling, Svensson, Morganti-Kossmann, Bellander et al.: "Lesion Size Is Exacerbated in Hypoxic Rats Whereas Hypoxia-Inducible Factor-1 Alpha and Vascular Endothelial Growth Factor Increase in Injured Normoxic Rats: A Prospective Cohort Study of Secondary ..." in: Frontiers in neurology, Vol. 7, pp. 23, (2016) (PubMed).
抗原

VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

别名

Vascular Endothelial Growth Factor A (VEGFA 产品)

别名

MVCD1 antibody, VEGF antibody, VPF antibody, Vegf antibody, Vegf120 antibody, Vegf164 antibody, Vegf188 antibody, Vpf antibody, vegf antibody, vegfa antibody, wu:fj82c06 antibody, VEGF-A antibody, VEGF164 antibody, eVEGF120 antibody, eVEGF164 antibody, vegf-a antibody, vpf antibody, vefg antibody, si:dkey-14d8.3 antibody, wu:fd42e02 antibody, vascular endothelial growth factor A antibody, vascular endothelial growth factor Aa antibody, vascular endothelial growth factor A L homeolog antibody, vascular endothelial growth factor Ab antibody, VEGFA antibody, Vegfa antibody, vegfaa antibody, vegfa.L antibody, vegfa antibody, vegfab antibody

背景

VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor

途径

RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling