WNT9A 抗体 (Internal Region)
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- 抗原 See all WNT9A 抗体
- WNT9A (Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This WNT9A antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human WNT9A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SCUBE2 (53 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey, Bat (94%) Mouse, Rat, Hamster, Bovine, Rabbit (88%) Elephant, Dog, Horse (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from internal region of human WNT9A. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey, Bat (94%), Mouse, Rat, Hamster, Bovine, Rabbit (88%), Elephant, Dog, Horse (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product WNT9A Primary Antibody
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anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) antibody
WNT9A 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (AA 206-365) antibodyWNT9A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (AA 21-120) antibodyWNT9A 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (Internal Region) antibodyWNT9A 适用: 人, Gorilla IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) antibodyWNT9A 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (C-Term) antibodyWNT9A 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (N-Term) antibodyWNT9A 适用: 人, 猴, Gorilla, Gibbon IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (AA 206-365) antibodyWNT9A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (AA 216-244) antibodyWNT9A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- WNT9A (Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A))
- 别名
- WNT14 / WNT9A (WNT9A 产品)
- 别名
- zgc:153617 antibody, wnt-9a antibody, Wnt14 antibody, WNT14 antibody, WNT-14 antibody, Wnt family member 9A antibody, wingless-type MMTV integration site family, member 9A antibody, wingless-type MMTV integration site family member 9A S homeolog antibody, WNT9A antibody, wnt9a antibody, wnt9a.S antibody, Wnt9a antibody
- 背景
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Name/Gene ID: WNT9A
Family: WNT
Synonyms: WNT9A, Protein Wnt-14, Protein Wnt-9a, WNT14 - 基因ID
- 7483
- 途径
- WNT signaling
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