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SEMA4B 抗体 (N-Term)

SEMA4B 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1049324
发货至: 中国
  • 抗原 See all SEMA4B 抗体
    SEMA4B (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4B (SEMA4B))
    抗原表位
    • 15
    • 7
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    • 1
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    N-Term
    适用
    • 50
    • 11
    • 5
    • 2
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    • 2
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    宿主
    • 48
    • 4
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    • 1
    克隆类型
    • 54
    • 3
    多克隆
    标记
    • 21
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    • 5
    • 2
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    • 2
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    This SEMA4B antibody is un-conjugated
    应用范围
    • 29
    • 18
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    • 1
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    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human SEMA4B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    预测反应
    Percent identity with other species by BLAST analysis: Human (100%) Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (94%) Marmoset, Elephant, Horse (88%) Dog (81%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 16 amino acid peptide from N-Terminus of human SEMA4B. Percent identity with other species by BLAST analysis: Human (100%), Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (94%), Marmoset, Elephant, Horse (88%), Dog (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product SEMA4B Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (10 - 15 μg/mL)
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    SEMA4B (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4B (SEMA4B))
    别名
    SEMA4B (SEMA4B 产品)
    别名
    sema4b antibody, MGC80974 antibody, SEMA4B antibody, SEMAC antibody, SemC antibody, Semac antibody, mKIAA1745 antibody, semaphorin 4B antibody, semaphorin 4B S homeolog antibody, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B antibody, SEMA4B antibody, sema4b.S antibody, Sema4b antibody
    背景
    Name/Gene ID: SEMA4B

    Synonyms: SEMA4B, KIAA1745, SEMAC, Semaphorin-4B, SemC
    基因ID
    10509
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