PTPRA 抗体 (Extracellular Domain)
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- 抗原 See all PTPRA 抗体
- PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))
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抗原表位
- Extracellular Domain
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PTPRA antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human PTPRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HLA-S (59 % ).
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Rat (94%) Hamster, Dog, Bovine, Rabbit (88%) Mouse, Panda, Horse (82%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 17 amino acid peptide from extracellular domain of human PTPRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Rat (94%), Hamster, Dog, Bovine, Rabbit (88%), Mouse, Panda, Horse (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product PTPRA Primary Antibody
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anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) (AA 20-142) antibody
PTPRA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) antibodyPTPRA 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) (Cytoplasmic Domain) antibodyPTPRA 适用: 人, 小鼠, 犬, Cow, Pig, 猴, 兔, 马, Bat, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) (Cytoplasmic Domain) antibodyPTPRA 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Cow, Pig, 猴, 兔, 斑马鱼, 马, Bat, Hamster, 非洲爪蟾 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) (AA 151-250) antibodyPTPRA 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) (AA 20-142) antibodyPTPRA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) (N-Term) antibodyPTPRA 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) (AA 618-796) antibodyPTPRA 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) antibodyPTPRA 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, A (PTPRA) (AA 686-735) antibodyPTPRA 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Cow, Pig, 猴, 兔, 斑马鱼, 马, Bat, 豚鼠, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IHC, IHC-P (15 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL. - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))
- 别名
- PTPRA / RPTP-alpha (PTPRA 产品)
- 别名
- PTPRA antibody, heptp antibody, hlpr antibody, hptpa antibody, hptpalpha antibody, lrp antibody, ptpa antibody, ptprl2 antibody, r-ptp-alpha antibody, rptpa antibody, HEPTP antibody, HLPR antibody, HPTPA antibody, HPTPalpha antibody, LRP antibody, PTPA antibody, PTPRL2 antibody, R-PTP-alpha antibody, RPTPA antibody, Ptpa antibody, Ptpalpha antibody, Rptpalpha antibody, Rptra antibody, Rptralpha antibody, RPTP[a] antibody, zf-RPTP[a] antibody, ptpa-a antibody, protein tyrosine phosphatase, receptor type A antibody, protein tyrosine phosphatase, receptor type, A antibody, protein tyrosine phosphatase, receptor type A S homeolog antibody, PTPRA antibody, ptpra antibody, Ptpra antibody, ptpra.S antibody
- 背景
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Name/Gene ID: PTPRA
Subfamily: R4
Family: Protein Phosphatase
Synonyms: PTPRA, HLPR, Hptp alpha, HEPTP, HPTPA, PTPA, Ptpalpha, PTPRL2, PTP alpha, PTPLCA-related phosphatase, R-PTP-alpha, RPTPA, Rptpalpha, Tyrosine phosphatase alpha, RPTP alpha, HPTPalpha, LRP, PTPase-alpha - 基因ID
- 5786
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