NR6A1 抗体 (Ligand Binding Domain)
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- 抗原 See all NR6A1 抗体
- NR6A1 (Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1))
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抗原表位
- Ligand Binding Domain
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适用
- 人, 小鼠, 大鼠, 兔, 犬, Pig, Cow, 马, 猴, Bat, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NR6A1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human NR6A1 / GCNF. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%) Platypus (89%) Turkey, Chicken (83%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 18 amino acid peptide from ligand-binding domain of human NR6A1 / GCNF. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%), Platypus (89%), Turkey, Chicken (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR6A1 Primary Antibody
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anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (AA 65-118) antibody
NR6A1 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4G8 unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) antibodyNR6A1 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 4G8 unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (AA 1-475) antibodyNR6A1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (AA 1-475) antibodyNR6A1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (Ligand Binding Domain) antibodyNR6A1 适用: 人, 小鼠, 大鼠, 兔, 犬, Pig, Cow, 马, 猴, Bat, Hamster, 小鸡 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (AA 323-372) antibodyNR6A1 适用: 人, 小鼠, 大鼠, 兔, 犬, Pig, 豚鼠, Cow, 马, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (AA 35-84) antibodyNR6A1 适用: 人, 小鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (AA 303-465) antibodyNR6A1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (C-Term) antibodyNR6A1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) (AA 282-480) antibodyNR6A1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (10 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- NR6A1 (Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1))
- 别名
- NR6A1 / GCNF (NR6A1 产品)
- 别名
- GCNF antibody, GCNF1 antibody, NR61 antibody, RTR antibody, hGCNF antibody, hRTR antibody, 1700113M01Rik antibody, Gcnf antibody, NCNF antibody, gcnf antibody, xEmGCNF antibody, gcnf1 antibody, nr61 antibody, rtr antibody, xGCNF antibody, NR6A1 antibody, GCNF-B antibody, si:ch211-167p9.1 antibody, zC167P9.1 antibody, zgc:114189 antibody, nr6a1 antibody, zfGCNF antibody, nuclear receptor subfamily 6 group A member 1 antibody, nuclear receptor subfamily 6, group A, member 1 antibody, nuclear receptor subfamily 6 group A member 1 S homeolog antibody, nuclear receptor subfamily 6, group A, member 1b antibody, nuclear receptor subfamily 6, group A, member 1a antibody, NR6A1 antibody, Nr6a1 antibody, nr6a1.S antibody, nr6a1 antibody, nr6a1b antibody, nr6a1a antibody
- 背景
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Name/Gene ID: NR6A1
Subfamily: NR6 Germ cell nuclear factor-like
Family: NHR
Synonyms: NR6A1, Germ cell nuclear factor, HGCNF, NR61, HRTR, RTR, Ncnf, GCNF, GCNF1 - 基因ID
- 2649
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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