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NEK9 抗体 (Kinase Domain)

NEK9 适用: 人, 小鼠, 马, 兔, Cow, Pig, 猴, Bat, 小鸡 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1049092
发货至: 中国
  • 抗原 See all NEK9 抗体
    NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))
    抗原表位
    • 10
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Kinase Domain
    适用
    • 77
    • 50
    • 27
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    人, 小鼠, 马, 兔, Cow, Pig, 猴, Bat, 小鸡
    宿主
    • 73
    • 4
    克隆类型
    • 76
    • 1
    多克隆
    标记
    • 32
    • 7
    • 7
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This NEK9 antibody is un-conjugated
    应用范围
    • 62
    • 38
    • 19
    • 13
    • 10
    • 7
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human NEK9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    预测反应
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard (100%) Rat, Dog (95%) Xenopus (90%) Pufferfish (80%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 20 amino acid peptide from kinase domain of human NEK9. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard (100%), Rat, Dog (95%), Xenopus (90%), Pufferfish (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product NEK9 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (4 μg/mL)
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))
    别名
    NEK9 (NEK9 产品)
    别名
    NERCC antibody, NERCC1 antibody, Nek8 antibody, NEK9 antibody, nercc antibody, nercc1 antibody, C130021H08Rik antibody, NIMA related kinase 9 antibody, NIMA-related kinase 9 antibody, NIMA related kinase 8 antibody, NIMA (never in mitosis gene a)-related expressed kinase 9 antibody, NIMA-related kinase 9 L homeolog antibody, NEK9 antibody, nek9 antibody, NEK8 antibody, Nek9 antibody, nek9.L antibody
    背景
    Name/Gene ID: NEK9
    Subfamily: NEK
    Family: Protein Kinase

    Synonyms: NEK9, KIAA1995, NERCC, NERCC1, Nercc1 kinase, NIMA-related kinase 8, NIMA-related kinase Nek8, NIMA-related kinase 9, NimA-related protein kinase 9
    基因ID
    91754
    途径
    SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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