KCNH2 抗体 (Internal Region)
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- 抗原 See all KCNH2 抗体
- KCNH2 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2))
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抗原表位
- Internal Region
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适用
- 人, 大鼠, 小鼠, 犬, 兔, Cow, 马, 猴, Pig, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This KCNH2 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human KCNH2 / Kv11.1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Bat, Guinea pig (94%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from internal region of human KCNH2 / Kv11.1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Bat, Guinea pig (94%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product KCNH2 Primary Antibody
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anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) antibody
KCNH2 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) (AA 430-445), (Extracellular) antibodyKCNH2 适用: 人, 大鼠 WB, IHC, FACS, IF, ICC, IP, LCI 宿主: 兔 Polyclonal unconjugated
anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) (AA 894-1137) antibodyKCNH2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) (AA 451-500) antibodyKCNH2 适用: 人, 小鼠, 兔, Cow, 马, 豚鼠, 绵羊 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) (Internal Region) antibodyKCNH2 适用: 人, 大鼠, 小鼠, 猴, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) (AA 1106-1159), (C-Term), (Intracellular) antibodyKCNH2 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) antibodyKCNH2 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) (C-Term) antibodyKCNH2 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2) (AA 895-944) antibodyKCNH2 适用: 人, 大鼠, 小鼠, 犬, 兔, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- KCNH2 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 2 (KCNH2))
- 别名
- KCNH2 / HERG (KCNH2 产品)
- 别名
- ERG1 antibody, HERG antibody, HERG1 antibody, Kv11.1 antibody, LQT2 antibody, SQT1 antibody, erg antibody, KCNH2 antibody, ERG antibody, gp-erg antibody, cerg antibody, derg antibody, erg1 antibody, AI326795 antibody, LQT antibody, Lqt2 antibody, M-erg antibody, Merg1 antibody, merg1a antibody, merg1b antibody, potassium voltage-gated channel subfamily H member 2 antibody, potassium voltage-gated channel, subfamily H (eag-related), member 6a antibody, potassium voltage-gated channel, subfamily H (eag-related), member 2 antibody, potassium voltage-gated channel subfamily H member 6 antibody, KCNH2 antibody, kcnh6a antibody, Kcnh2 antibody, KCNH6 antibody
- 背景
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Name/Gene ID: KCNH2
Subfamily: Potassium channel - Eag-related
Family: Ion Channel
Synonyms: KCNH2, Eag homolog, HERG-1, ERG1, HERG, KV11.1, M-erg1, H-ERG, HERG1, SQT1, LQT2, Merg1, Eag-related protein 1, ERG-1 - 基因ID
- 3757
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