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- 抗原 See all CEACAM1 抗体
- CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CEACAM1 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CEACAM1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Orangutan, Gibbon (92%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 13 amino acid peptide from internal region of human CEACAM1. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Orangutan, Gibbon (92%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CEACAM1 Primary Antibody
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anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) antibody
CEACAM1 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) antibodyCEACAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) (AA 65-201) antibodyCEACAM1 适用: 人 WB, IHC, FACS, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 4F3B2 unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) (AA 300-430) antibodyCEACAM1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) antibodyCEACAM1 适用: 人 FACS, IHC (fro), cELISA 宿主: 小鼠 Monoclonal GM-8G5 unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) (AA 35-143) antibodyCEACAM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) (AA 36-145) antibodyCEACAM1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) (AA 1-468) antibodyCEACAM1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) (AA 65-201) antibodyCEACAM1 适用: 人 WB, IHC, FACS, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 4H8C9 unconjugated
anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) antibodyCEACAM1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))
- 别名
- CD66A / CEACAM1 (CEACAM1 产品)
- 别名
- BGP antibody, BGP1 antibody, BGPI antibody, CD66e antibody, CEA antibody, zeh0068 antibody, hm:zeh0068 antibody, CEACAM8 antibody, CEACAM1 antibody, MGC142545 antibody, BGPR antibody, Bgp antibody, CD66a antibody, Ccam1 antibody, Bgp1 antibody, C-CAM antibody, Cc1 antibody, Cea-1 antibody, Cea-7 antibody, Cea1 antibody, Cea7 antibody, Hv-2 antibody, Hv2 antibody, MHVR antibody, MHVR1 antibody, Mhv-1 antibody, bb-1 antibody, mCEA1 antibody, mmCGM1 antibody, mmCGM1a antibody, mmCGM2 antibody, carcinoembryonic antigen related cell adhesion molecule 1 antibody, carcinoembryonic antigen related cell adhesion molecule 5 antibody, carcinoembryonic antigen-related cell adhesion molecule 1 antibody, carcinoembryonic antigen-related cell adhesion molecule 6 antibody, CEACAM1 antibody, CEACAM5 antibody, ceacam1 antibody, Ceacam1 antibody, LOC101103343 antibody
- 背景
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Name/Gene ID: CEACAM1
Family: CEA
Synonyms: CEACAM1, Antigen CD66, BGP1, Biliary glycoprotein 1, CD66a, BGP-1, BGP, BGPI, CD66a antigen - 基因ID
- 634
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