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Angiotensin II Type 2 Receptor 抗体 (Internal Region)

AGTR2 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 犬, 猴, Hamster, 绵羊, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1048323
发货至: 中国
  • 抗原 See all Angiotensin II Type 2 Receptor (AGTR2) 抗体
    Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))
    抗原表位
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    适用
    • 45
    • 34
    • 32
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, Cow, 马, Pig, 犬, 猴, Hamster, 绵羊, Bat
    宿主
    • 42
    • 4
    • 1
    克隆类型
    • 32
    • 15
    多克隆
    标记
    • 18
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Angiotensin II Type 2 Receptor antibody is un-conjugated
    应用范围
    • 32
    • 10
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human AGTR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR65 (63 % ), NPFFR2 (44 %).
    预测反应
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 16 amino acid peptide from internal region of human AGTR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product AGTR2 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (10 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))
    别名
    AGTR2 / AT2 Receptor (AGTR2 产品)
    别名
    AT2 antibody, ATGR2 antibody, MRX88 antibody, AI316812 antibody, AW107640 antibody, AT2-R antibody, AT2R antibody, AT2R antibody, at2 antibody, zgc:113016 antibody, AGTR2 antibody, angiotensin II receptor type 2 antibody, angiotensin II receptor, type 2 antibody, AGTR2 antibody, Agtr2 antibody, agtr2 antibody
    背景
    Name/Gene ID: AGTR2
    Subfamily: Angiotensin
    Family: GPCR

    Synonyms: AGTR2, Angiotensin ii receptor type 2, Angiotensin II type-2 receptor, AT2 receptor, AT2, At2r, MRX88, Angiotensin ii at2, Angiotensin receptor 2, Type-2 angiotensin II receptor, ATGR2
    基因ID
    186
    途径
    ACE Inhibitor Pathway, Hormone Transport, Regulation of Systemic Arterial Blood Pressure by Hormones
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