ACVR1C/ALK7 抗体 (Internal Region)
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- 抗原 See all ACVR1C/ALK7 (ACVR1C) 抗体
- ACVR1C/ALK7 (ACVR1C) (Activin Receptor Type 1C (ACVR1C))
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抗原表位
- Internal Region
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适用
- 人, Cow, Pig, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ACVR1C/ALK7 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ACVR1C. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Bat, Pig (100%) Marmoset, Elephant, Panda, Horse, Rabbit (94%) Opossum, Turkey, Chicken, Platypus (88%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from internal region of human ACVR1C. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Bovine, Bat, Pig (100%), Marmoset, Elephant, Panda, Horse, Rabbit (94%), Opossum, Turkey, Chicken, Platypus (88%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ACVR1C Primary Antibody
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anti-Activin Receptor Type 1C (ACVR1C) (AA 21-120) antibody
ACVR1C 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Activin Receptor Type 1C (ACVR1C) (AA 22-113) antibodyACVR1C 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Activin Receptor Type 1C (ACVR1C) antibodyACVR1C 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Activin Receptor Type 1C (ACVR1C) (N-Term) antibodyACVR1C 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Activin Receptor Type 1C (ACVR1C) antibodyACVR1C 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Activin Receptor Type 1C (ACVR1C) (C-Term) antibodyACVR1C 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Activin Receptor Type 1C (ACVR1C) (Center) antibodyACVR1C 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Activin Receptor Type 1C (ACVR1C) (AA 21-493) antibodyACVR1C 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activin Receptor Type 1C (ACVR1C) (AA 33-62), (N-Term) antibodyACVR1C 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB8195 unconjugated
anti-Activin Receptor Type 1C (ACVR1C) (AA 72-121) antibodyACVR1C 适用: 人, 大鼠, Cow, 兔, 豚鼠, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL)
Usage: Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K. - 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- ACVR1C/ALK7 (ACVR1C) (Activin Receptor Type 1C (ACVR1C))
- 别名
- ACVR1C / ALK7 (ACVR1C 产品)
- 别名
- ACVRLK7 antibody, ALK7 antibody, Alk-7 antibody, C230097P10 antibody, Alk7 antibody, habrec1 antibody, activin A receptor type 1C antibody, activin A receptor, type IC antibody, ACVR1C antibody, Acvr1c antibody
- 背景
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Name/Gene ID: ACVR1C
Subfamily: Activin/TGFb
Family: Protein Kinase
Synonyms: ACVR1C, Activin receptor-like kinase 7, ACTR-IC, Activin receptor type IC, ACVRLK7, Activin receptor type-1C, ALK7, Activin A receptor, type IC, ALK-7 - 基因ID
- 130399
- 途径
- Negative Regulation of Hormone Secretion, Positive Regulation of Endopeptidase Activity
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