NYS48/HAUS8 抗体 (Internal Region)
(1 reference)-
- 抗原 See all NYS48/HAUS8 (HAUS8) 抗体
- NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NYS48/HAUS8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Anti-Hice1 Affinity purified antibody is directed against human Hice1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
- 产品特性
- Hice1 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gammaTuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
- 过滤
- Sterile filtered
- 免疫原
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Anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1.
Immunogen Type: Peptide - 亚型
- IgG
- Top Product
- Discover our top product HAUS8 Primary Antibody
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anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) antibody
KO Validated HAUS8 适用: 人 WB, IF, IHC (p), ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (AA 166-410) antibodyHAUS8 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (AA 1-248) antibodyHAUS8 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) antibodyHAUS8 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) antibodyHAUS8 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (AA 57-106) antibodyHAUS8 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (N-Term) antibodyHAUS8 适用: 人, Pig WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Hice1 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
- 说明
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Gene Name: Hice1
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.45 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3652-62, (2008) (PubMed).
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: "Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3652-62, (2008) (PubMed).
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- 抗原
- NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
- 别名
- HICE1 (HAUS8 产品)
- 别名
- 2410004L22Rik antibody, Hice1 antibody, NY-SAR-48 antibody, hice1 antibody, DGT4 antibody, HICE1 antibody, Ny-sar-48 antibody, RGD1311565 antibody, 4HAUS augmin-like complex, subunit 8 antibody, HAUS augmin like complex subunit 8 L homeolog antibody, HAUS augmin like complex subunit 8 antibody, HAUS augmin-like complex, subunit 8 antibody, Haus8 antibody, haus8.L antibody, HAUS8 antibody
- 背景
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Hice1 is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the γTuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
Synonyms: HAUS8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48 - 基因ID
- 93323
- NCBI登录号
- NP_219485
- UniProt
- Q9BT25
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