AKT3 抗体 (Internal Region)
(1 reference)-
- 抗原 See all AKT3 抗体
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This AKT3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
- 产品特性
- AKT3 Antibody detects AKT3 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT3 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
- 过滤
- Sterile filtered
- 免疫原
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Anti-AKT3 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
Immunogen Type: Peptide - 克隆位点
- 25F6-F6-D8
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product AKT3 Primary Antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 100-189) antibody
AKT3 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 6E11 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (Internal Region) antibody (FITC)AKT3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS, FM 宿主: 小鼠 Monoclonal 25F6-F6-D8 FITC
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 46-338) antibodyAKT3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 119-133) antibodyVerified AKT3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (Internal Region) antibody (Biotin)AKT3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS, FM 宿主: 小鼠 Monoclonal 25F6-F6-D8 Biotin
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 37-150) antibodyAKT3 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5H7C3 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (Internal Region) antibody (PE)AKT3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS, FM 宿主: 小鼠 Monoclonal 25F6-F6-D8 PE
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 100-189) antibodyAKT3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6E10 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) (AA 37-150) antibodyAKT3 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 7B7A2 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) antibodyAKT3 适用: 人, 小鼠 WB, IHC, FACS, IF 宿主: 小鼠 Monoclonal 9B2 unconjugated
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- 应用备注
- Anti-AKT3 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
- 说明
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Gene Name: AKT3
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "Glutamatergic neurotransmission in the synapsin I and II double knock-out mouse." in: Seminars in cell & developmental biology, Vol. 22, Issue 4, pp. 400-7, (2011) (PubMed).
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: "Glutamatergic neurotransmission in the synapsin I and II double knock-out mouse." in: Seminars in cell & developmental biology, Vol. 22, Issue 4, pp. 400-7, (2011) (PubMed).
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- 抗原
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
- 别名
- AKT3 (AKT3 产品)
- 别名
- MPPH antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AI851531 antibody, D930002M15Rik antibody, Nmf350 antibody, Pkbg antibody, AKT serine/threonine kinase 3 antibody, thymoma viral proto-oncogene 3 antibody, v-akt murine thymoma viral oncogene homolog 3b antibody, AKT3 antibody, Akt3 antibody, akt3b antibody
- 背景
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AKT3 Antibody detects AKT3 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT3 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
Synonyms: AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma - 基因ID
- 10000
- UniProt
- Q9Y243
- 途径
- PI3K-Akt Signaling, RTK signaling, TLR signaling, Hepatitis C, VEGF Signaling
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