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兔 anti-小鼠 IgM (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
产品编号 ABIN102683
发货至: 中国
  • 抗原 See all IgM products
    IgM
    抗原表位
    • 141
    • 118
    • 87
    • 40
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy & Light Chain
    适用
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    小鼠
    宿主
    • 321
    • 187
    • 73
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    克隆类型
    • 495
    • 78
    多克隆
    标记
    • 119
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    • 1
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    Alkaline Phosphatase (AP)
    应用范围
    • 332
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    IgM µ chain
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    纯化方法
    Preadsorption: Solid phase absorption
    过滤
    Sterile filtered
    免疫原

    Immunogen: Mouse IgM whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-MOUSE IgM (alpha chain) (RABBIT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

    ELISA Dilution: 1:20,000

    Western Blot Dilution: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    储存条件
    4 °C
    有效期
    12 months
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit Anti-Mouse IgM (mu chain) Antibody alkaline phosphatase conjugated, Rabbit Anti-Mouse IgM mu chain Antibody alk phos Conjugation

    Background: MOUSE IgM (alpha chain) (RABBIT) Antibody generated in rabbit detects specifically mouse IgM whole molecule. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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